Evidence map›Paper›PMID 42129049›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Methods for Autophagy Detection by Fluorescence Microscopy.

Xiaoye Ke, Xianglong Zhang, Xiangxiang Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Xiaoye KePepper Germplasm Research Group, College of Agriculture, Anhui Science and Technology University, Chuzhou, Anhui, P. R. China.
Xianglong ZhangPepper Germplasm Research Group, College of Agriculture, Anhui Science and Technology University, Chuzhou, Anhui, P. R. China.
Xiangxiang ZhangPepper Germplasm Research Group, College of Agriculture, Anhui Science and Technology University, Chuzhou, Anhui, P. R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescence microscopy is pivotal for investigating autophagy's role in plant antiviral immunity. Here, we present a standardized procedure using complementary probes, CFP-ATG8f for autophagosomal structures and monodansylcadaverine (MDC) for autophagic vacuoles, to assess autophagy during viral infection. This combined CFP-ATG8f and MDC staining system provides a powerful, reproducible method for evaluating autophagic activity in plant-virus interactions.

Indexed as

AutophagyArabidopsisArabidopsis ProteinsAutophagy-Related Protein 8 FamilyCadaverineMicroscopy, ConfocalMicroscopy, FluorescencePlant DiseasesStaining and LabelingArabidopsis ProteinsAutophagy-Related Protein 8 FamilyCadaverinemonodansylcadaverineAutophagyCFP-ATG8fConfocal microscopyMDC staining

Identifiers

PMID42129049

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.