ArticleMethods in molecular biology (Clifton, N.J.)2026
High-Throughput Screening of Antiviral Agents Using the Fluorescence-Based Local Lesion Inspection Assay.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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6 authors.
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Abstract
A high-throughput antiviral agent screening system called the fluorescence-based local lesion inspection assay (FLLIA) has been developed. In this assay, leaves were mechanically inoculated with an engineered virus expressing a fluorescent protein. One half of each leaf was treated with a test chemical and the other half was treated with a buffer solution. The number and size of viral infection lesions were then imaged and quantified. A reduction in the number of viral infection lesions and/or decreased fluorescence intensity in the chemically treated group suggests a replication inhibition activity, while a reduction in the size of fluorescent lesions indicates a movement inhibition activity. This simple method can efficiently identify antiviral agents and primarily differentiate their action mechanisms. A step-by-step protocol of FLLIA was provided in this chapter.
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