Evidence map›Paper›PMID 42129044›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

VIGS-Based Screening for NLR Genes Regulating Viral Effector-Induced Hypersensitive Response.

Wenqi Mao, Jianping Chen, Fei Yan, Guanwei Wu

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Wenqi MaoState Key Laboratory for Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China.
Jianping ChenState Key Laboratory for Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China.
Fei YanState Key Laboratory for Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China. yanfei@nbu.edu.cn.
Guanwei WuState Key Laboratory for Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China. wuguanwei@nbu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nucleotide-binding leucine-rich repeat protein (NLR) can specifically recognize effector proteins secreted by pathogens, thereby initiating a strong immune response in host plants. Currently, utilizing this resistance gene to improve crop varieties is the most economically effective strategy. However, traditional identification methods are time-consuming and labor-intensive. RNA interference (RNAi) is a sequence-specific gene-silencing mechanism mediated by double-stranded RNA. This chapter provides a detailed protocol using tobacco rattle virus-mediated virus-induced gene-silencing (VIGS) screening platform in Nicotiana benthamiana for the fast identification of host NLR gene involved in viral effector-activated hypersensitive response (HR), with the turnip mosaic virus-coded nuclear inclusion protein a protease (NIa-Pro) as an example, which has been shown to activate HR in Nicotiana benthamiana.

Indexed as

NicotianaNLR ProteinsPlant DiseasesPlant ProteinsPlant VirusesGene Expression Regulation, PlantGene SilencingHost-Pathogen InteractionsPotyvirusRNA InterferenceViral ProteinsNLR ProteinsPlant ProteinsViral ProteinsHypersensitive responseNucleotide-binding leucine-rich repeat proteinTobacco rattle virusVirus-induced gene silencing

Identifiers

PMID42129044

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.