Evidence map›Paper›PMID 42129034›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Construction of Attenuated Viruses by Changing the UpA Dinucleotide Frequency.

Shuaifa Chang, Yong Li, Yixin Huang, Bo Song, Adrian Valli, Xiaoyun Wu, Xiaofei Cheng, Weiqin Ji

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shuaifa ChangCollege of Plant Protection, Northeast Agricultural University, Harbin, Heilongjiang, P. R. China.
Yong LiCollege of Life Science, Northeast Agricultural University, Harbin, Heilongjiang, P. R. China.
Yixin HuangCollege of Plant Protection, Northeast Agricultural University, Harbin, Heilongjiang, P. R. China.
Bo SongCollege of Plant Protection, Northeast Agricultural University, Harbin, Heilongjiang, P. R. China.
Adrian ValliCentro Nacional de Biotecnología, CSIC, Madrid, Spain.
Xiaoyun WuCollege of Plant Protection, Northeast Agricultural University, Harbin, Heilongjiang, P. R. China.
Xiaofei ChengCollege of Plant Protection, Northeast Agricultural University, Harbin, Heilongjiang, P. R. China.ORCID https://orcid.org/0000-0002-9771-9059
Weiqin JiCollege of Plant Protection, Northeast Agricultural University, Harbin, Heilongjiang, P. R. China. jiweiqinppc@neau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cross protection with an attenuated virus is a promising strategy for controlling viral diseases in crops that lack resistance resources. Attenuated viruses are usually obtained from naturally avirulent strains or through mutagenesis. Studies have shown that increasing the UpA dinucleotide content in the genome without altering the amino acid encoding sequence causes the loss of virulence. This chapter describes a detailed protocol for developing attenuated virus by increasing the UpA dinucleotide content, using turnip mosaic virus as a case study. This approach enables the rapid acquisition of stably, inheritable attenuated virus strains.

Indexed as

PotyvirusGenome, ViralPlant DiseasesVaccines, AttenuatedVirulenceVaccines, AttenuatedAttenuated strainCross-protectionTuMVUpAUpA dinucleotide

Identifiers

PMID42129034

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.