Evidence map›Paper›PMID 42129030›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Development of Tobamovirus-Resistant Tomato Plants by CRISPR-Cas9-Mediated Knockout of Susceptibility Genes.

Kikyo Watanabe, Masayuki Ishikawa, Kazuhiro Ishibashi

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Kikyo WatanabeInstitute of Agrobiological Sciences, National Agriculture and Food Research Organizations (NARO), Tsukuba, Ibaraki, Japan.
Masayuki IshikawaInstitute of Agrobiological Sciences, National Agriculture and Food Research Organizations (NARO), Tsukuba, Ibaraki, Japan.
Kazuhiro IshibashiInstitute of Agrobiological Sciences, National Agriculture and Food Research Organizations (NARO), Tsukuba, Ibaraki, Japan. ishibashi.kazuhiro486@naro.go.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The advent of genome editing technologies such as CRISPR-Cas9 has revolutionized the development of disease-resistant crops, offering precision and efficiency in targeting specific genetic loci responsible for susceptibility. In this protocol, we harness the CRISPR-Cas9 system to disrupt key susceptibility genes in tomato, aiming to fortify resistance against tobamoviruses, particularly the aggressive tomato brown rugose fruit virus (ToBRFV). By systematically knocking out four TOM1 homologs, genes essential for tobamoviral replication, tomato lines with robust and heritable resistance can be developed while minimizing adverse developmental effects. The approach not only underscores the significance of basic research on host-pathogen interactions in modern crop protection but also lays the groundwork for sustainable, gene-driven resistance strategies in commercial tomato breeding.

Indexed as

CRISPR-Cas SystemsDisease ResistanceGene Knockout TechniquesPlant DiseasesSolanum lycopersicumTobamovirusGene EditingHost-Pathogen InteractionsPlant ProteinsPlants, Genetically ModifiedPlant ProteinsCRISPR-Cas9Disease resistanceGenome editingSusceptibility genesSustainable agricultureTobamovirusToBRFVTOM1Tomato

Identifiers

PMID42129030

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.