Homogeneous Antibody-DNA Conjugates Using Unmodified Oligonucleotides and Photo-Cross-Linkable Protein G-HUH Endonuclease Fusion Proteins.

Anna Swietlikowska, Femi Hesen, Alexander Gräwe, Maarten Merkx

Abstract read

In one paragraph

Article in Bioconjugate chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Anna SwietlikowskaLaboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, Eindhoven 5600 MB, The Netherlands.ORCID 0009-0008-7289-7642

Femi HesenLaboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, Eindhoven 5600 MB, The Netherlands.

Alexander GräweLaboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, Eindhoven 5600 MB, The Netherlands.

Maarten MerkxLaboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, Eindhoven 5600 MB, The Netherlands.ORCID 0000-0001-9484-3882

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antibody-DNA conjugates are increasingly used in analytical biochemistry and nanotechnology. However, current methods for their preparation often lack specificity, resulting in heterogeneous products. Here, we present a novel strategy to covalently label the Fc domain of antibodies with single-stranded DNA in a site-specific and stoichiometrically controlled manner. This method employs unmodified oligonucleotides and a fusion protein consisting of a protein G dimer linked to an HUH endonuclease. We first evaluated the sequence specificity and optimal conditions for DNA attachment to three HUH endonuclease variants: DCV, PCV2, and WDV. Our results show increased sequence specificity in the presence of Mg

Indexed as

Cross-Linking ReagentsDNAEndonucleasesOligonucleotidesRecombinant Fusion ProteinsBacterial ProteinsPhotochemical ProcessesBacterial ProteinsCross-Linking ReagentsDNAEndonucleasesIgG Fc-binding protein, StreptococcusOligonucleotidesRecombinant Fusion Proteins

Identifiers

PMID42128399

DOI10.1021/acs.bioconjchem.5c00566

PMCPMC13281382

What OpenQuestion holds

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LicenceCC BY-NC-ND

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.