ArticleAnimal bioscience2026
The effect of zinc selenide nanospheres on porcine semen cryopreservation.
Article in Animal bioscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
objectiveThis study aimed to investigate the protective effects of zinc selenide (ZnSe) nanospheres on boar semen during cryopreservation and to determine the optimal supplementation concentration for improving post-thaw sperm quality and antioxidant status.
methodsZnSe nanospheres were synthesized via a hydrothermal method using Na2SeO3 and Zn(CH3COO)2 as precursors. Ejaculates with motility ≥70% were collected from six healthy boars, pooled to minimize individual variation, and the entire experiment was independently repeated three times. Samples were mixed with cryopreservation extenders containing 0 (control), 0.1, 1, or 10 mmol/L ZnSe nanospheres. Post-freezing and thawing, sperm motility parameters, acrosome integrity, plasma membrane integrity, antioxidant enzyme activities, and intracellular reactive oxygen species (ROS) levels were evaluated.
resultsSupplementation with 1 mmol/L ZnSe significantly improved post-thaw sperm motility, acrosome integrity, and plasma membrane integrity (p<0.05). Moreover, 1 mmol/L ZnSe markedly enhanced total antioxidant capacity, catalase, and superoxide dismutase activities, while reducing malondialdehyde concentrations and intracellular ROS levels (p<0.05).
conclusionSupplementation with 1 mmol/L ZnSe in the cryopreservation diluent effectively improves post-thaw sperm quality and antioxidant capacity, offering a promising strategy for optimizing porcine semen cryopreservation.
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