ArticlePoultry science2026
Effects of fine-to-coarse limestone ratio on performance, egg quality, bone traits, and renal CYP27B1 protein abundance in peak and aged Lohmann Brown laying hens.
Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two trials were conducted to evaluate performance, egg quality, blood and bone traits, and renal 1α-hydroxylase (CYP27B1) protein abundance in laying hens fed different fine-to-coarse limestone (FL:CL) ratios during distinct production phases. A total of 180 Lohmann Brown hens at 26 weeks of age (Trial 1) and 96 hens at 66 weeks of age (Trial 2) were assigned to dietary FL:CL ratios of 60:40, 50:50, and 30:70 in Trial 1 and 50:50 and 30:70 in Trial 2. In both trials, dietary FL:CL ratios did not affect performance, egg quality, or blood parameters. In Trial 1, the 30:70 FL:CL ratio reduced relative tibia weight and tibia breaking strength (p < 0.05), whereas hens fed the 50:50 ratio exhibited approximately 20 % lower renal CYP27B1 protein abundance (p < 0.05). In Trial 2, the 30:70 ratio increased feed intake (p < 0.05) and reduced tibial Ca content (p < 0.05), while other variables remained unaffected. Overall, altering FL:CL ratios had no effect on eggshell quality and had limited effects on productive performance, but higher proportions of coarse limestone negatively affected specific bone traits. In addition, the intermediate 50:50 FL:CL ratio was associated with lower renal CYP27B1 protein abundance, which is consistent with a reduced demand for vitamin D
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.