ArticlePoultry science2026
Rapid visual detection of Mycoplasma gallisepticum by combining recombinase-aided amplification with lateral-flow dipstick assay.
Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Development and evaluation of a recombinase-aided amplification-lateral flow dipstick assay for rapid and sensitive detection ofFrontiers in cellular and infection microbiology · 2026Article
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7 authors.
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Abstract
Mycoplasma gallisepticum (MG) is a significant avian pathogen that adversely affects chickens and turkeys globally and is classified as a notifiable disease agent by the World Organisation for Animal Health. Therefore, rapid and accurate diagnosis is a critical factor in controlling MG infections. This study established a simple, rapid, and visual detection method for MG using recombinase-aided amplification (RAA) combined with a lateral flow dipstick (LFD). Following primer and probe screening, the RAA-LFD method was optimized for primer concentration, probe concentration, reaction temperature, and time, and its specificity and sensitivity were analyzed. The results demonstrated that under isothermal conditions at 39°C, RAA could amplify the target gene within 20 minutes, and the products could be visualized via LFD within 5 minutes. The method showed no cross-reactivity with Mycoplasma synoviae, Mycoplasma bovis, Pasteurella multocida, Mannheimia haemolytica, Staphylococcus aureus, Salmonella spp., Escherichia coli, Listeria monocytogenes, Enterococcus faecium, or Enterococcus faecalis. Furthermore, the RAA-LFD method exhibited high sensitivity and good reproducibility, with a detection limit of 10 copies/μL. A total of 100 clinical samples suspected of MG infection were tested using RAA, PCR, and real-time fluorescence quantitative PCR (qPCR). The concordance rates between the RAA detection method and conventional PCR or qPCR were 96% and 98%, respectively. These results indicate that the established RAA-LFD method is easy to operate, highly specific, and sensitive, making it suitable for grassroots-level field diagnosis of MG infections.
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