ArticleSTAR protocols2026
Protocol for SUMO fusion-based functional rescue of SUMOylation-defective protein in Drosophila.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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4 authors.
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Abstract
Here, we present a protocol for the functional rescue of small ubiquitin-like modifier (SUMO)ylation-defective proteins using a SUMO fusion-based gain-of-function approach. Using Panx involved in the Piwi-piRNA-mediated genome defense in Drosophila, we describe the restoration of transcriptional silencing by stable fusion of a non-cleavable SUMO variant. We then detail procedures for the quantitative evaluation of transcriptional repression. This approach enables causal analysis of SUMO-dependent protein function beyond conventional loss-of-function strategies. For complete details of this protocol, please refer to Wu et al.
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