Evidence map›Paper›PMID 42126753›Full record

ArticleWorld journal of microbiology & biotechnology2026

Genetic Diversity analysis of industrial Saccharomyces cerevisiae strains and their correlation with fermentation performance.

Liping Lei, Qiuli Bin, Changhua Yu, Xuemei Qin, Xinran Shan, Renzhi Wu, Shuwei Cao

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Article in World journal of microbiology & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

7 authors.

Liping LeiGuangxi Key Laboratory of Polysaccharide Materials and Modification, School of Marine Sciences and Biotechnology, Guangxi-Indonesia Joint Laboratory for Microbial Resources and Artificial Intelligence, Guangxi Minzu University, 530008, Nanning, China.ORCID http://orcid.org/0009-0004-1707-7589
Qiuli BinGuangxi Key Laboratory of Polysaccharide Materials and Modification, School of Marine Sciences and Biotechnology, Guangxi-Indonesia Joint Laboratory for Microbial Resources and Artificial Intelligence, Guangxi Minzu University, 530008, Nanning, China.
Changhua YuAnimal Husbandry Research Institute, Guangxi Vocational University of Agriculture, 530007, Nanning, China.
Xuemei QinGuangxi Key Laboratory of Polysaccharide Materials and Modification, School of Marine Sciences and Biotechnology, Guangxi-Indonesia Joint Laboratory for Microbial Resources and Artificial Intelligence, Guangxi Minzu University, 530008, Nanning, China.
Xinran ShanGuangxi Key Laboratory of Polysaccharide Materials and Modification, School of Marine Sciences and Biotechnology, Guangxi-Indonesia Joint Laboratory for Microbial Resources and Artificial Intelligence, Guangxi Minzu University, 530008, Nanning, China.
Renzhi WuGuangxi Key Laboratory of Polysaccharide Materials and Modification, School of Marine Sciences and Biotechnology, Guangxi-Indonesia Joint Laboratory for Microbial Resources and Artificial Intelligence, Guangxi Minzu University, 530008, Nanning, China. rzwu0721@126.com.
Shuwei CaoAnimal Husbandry Research Institute, Guangxi Vocational University of Agriculture, 530007, Nanning, China. 743495959@qq.com.

Funding

National Natural Science Foundation of China 32260018the Autonomous Region College Student Innovation Training Program S202510608296the Graduate Education Innovation Program of Guangxi Minzu University gxmzu-chxs2024242, gxun-chxs2024113the National College Student Innovation Training Program 202410608024the Talent Introduction Project of Guangxi Minzu University 2022KJQD19the Xiangsihu Young Scholars Innovation Team of Guangxi Minzu University 2022GXUNXSHQN05
6 · The paper itself

Abstract

Sugarcane molasses serves as a vital non-food feedstock for ethanol fermentation, and its efficient utilisation depends on breeding of excellent microbial strains. Therefore, identification of industrial strains for ethanol fermentation is central to improving fermentation efficiency. To identify high-efficiency ethanol-fermenting strains, we developed a method for preparing high-throughput polymerase chain reaction (PCR) templates within 3 min using seven industrial Saccharomyces cerevisiae strains, integrating single-cell morphology and phenotypic variations in ethanol fermentation. Method specificity was confirmed by amplifying and sequencing the internal transcribed spacer region, and the efficiency was validated by extracting genomic DNA using cetyltrimethylammonium bromide (CTAB). Subsequent genetic diversity analysis using random amplified polymorphic DNA (RAPD) with eight effective primers screened from twenty-two random primers revealed genetic similarity coefficients ranging between 0.47 and 0.96. The high-yield strains 1015-04-01 and 1002-03-03 showed the highest similarity (0.96), whereas strain 1015-04-01 and the low-yield strain 1415 exhibited the lowest similarity (0.47). Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis demonstrated that the high-yield strain 1016-02-04, and strains 1415 and 1313 formed a major cluster, and the low-yield strains 1415 and 1313 formed a distinct subcluster; four other high-yield strains constituted a separate cluster, indicating certain genetic differences between high- and low-yield strains, together with partial genetic overlap. The RAPD results exhibited a certain correlation with differences in final ethanol concentration and single-cell morphology, providing preliminary genetic marker references for the rapid identification and screening of high-performance strains used in ethanol fermentation from sugarcane molasses.

Indexed as

FermentationGenetic VariationSaccharomyces cerevisiaeCluster AnalysisDNA, FungalEthanolIndustrial MicrobiologyMolassesPhylogenyPolymerase Chain ReactionRandom Amplified Polymorphic DNA TechniqueSaccharumSequence Analysis, DNADNA, FungalEthanolEthanol fermentationGenetic diversityRAPDYeast

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.