A New Fluorogenic Substrate for CYP1A2 and Its Application in Studying the Effects of Alcohol Exposure on Liver Drug Metabolism.

Kari Gaither, Nadezhda Davydova, Kannapiran Ponraj, Dilip K Singh, Bhagwat Prasad, Dmitri R Davydov

Abstract readPreprint

In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Kari GaitherDepartment of Chemistry, Washington State University, Pullman, WA, 99164.ORCID 0000-0003-2014-4362

Nadezhda DavydovaDepartment of Chemistry, Washington State University, Pullman, WA, 99164.

Kannapiran PonrajDepartment of Chemistry, Washington State University, Pullman, WA, 99164.

Dilip K SinghDivision of Translational and Clinical Pharmacology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, 45229.

Bhagwat PrasadDivision of Translational and Clinical Pharmacology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, 45229.ORCID 0000-0002-9090-0912

Dmitri R DavydovDepartment of Chemistry, Washington State University, Pullman, WA, 99164.ORCID 0000-0001-8520-0033

Funding

Inter-Enzyme Crosstalk in the Cytochrome P450 Ensemble: Implications for the Effects of Alcohol on Drug Metabolism and Alcohol-Drug InteractionsR01AA030155 · NIAAA · WASHINGTON STATE UNIVERSITY · PI DAVYDOV, DMITRI R, PRASAD, BHAGWAT · 2022 to 2025

$2.0M

NIAAA NIH HHS R01 AA030155

6 · The paper itself

Abstract

Aiming to develop a high-throughput fluorimetric assay for the activity CYP1A2, we introduced 6-Methoxy-2-naphthoic acid (MONA) as a new fluorogenic substrate for this important metabolizer of antidepressants and psychotropic drugs in human liver. We demonstrated that oxidative demethylation of MONA by liver microsomes results in a red shift and a substantial increase in fluorescence. This effect, which is exceptionally well pronounced at alkaline pH, allowed us to develop a sensitive and robust high-throughput assay of MONA metabolism. Probing the activity of 15 individual recombinant human P450 enzymes, we found that only two P450 species exhibited activity in MONA demethylation: CYP1A2 (

Indexed as

3-(6-Methoxynaphthalen-2-yl)acrylic acid6-methoxy-2-naphthoic acidCYP1A2CYP2A6cytochrome P450fluorogenic substrateshigh-throughput activity assayhuman liver microsomes

Identifiers

PMID42124730

DOI10.64898/2026.02.21.703381

PMCPMC13160161

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.