Evidence map›Paper›PMID 42124648›Full record

ArticlebioRxiv : the preprint server for biology2026

RNA-binding is the essential biological function of the

Robert P Connacher, Yichao Hu, Richard Roden, Julia Toledo, Anna DesMarais, Michael O'Connor, Howard D Lipshitz, Aaron C Goldstrohm

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Robert P ConnacherDepartment of Biochemistry, Molecular Biology, & Biophysics, University of Minnesota, USA.ORCID 0000-0003-4560-3852
Yichao HuDepartment of Molecular Genetics, University of Toronto, Canada.ORCID 0009-0001-2724-0253
Richard RodenDepartment of Biochemistry, Molecular Biology, & Biophysics, University of Minnesota, USA.
Julia ToledoDepartment of Biochemistry, Molecular Biology, & Biophysics, University of Minnesota, USA.
Anna DesMaraisDepartment of Biochemistry, Molecular Biology, & Biophysics, University of Minnesota, USA.
Michael O'ConnorDepartment of Genetics, Cell Biology, and Development, University of Minnesota, USA.
Howard D LipshitzDepartment of Molecular Genetics, University of Toronto, Canada.
Aaron C GoldstrohmDepartment of Biochemistry, Molecular Biology, & Biophysics, University of Minnesota, USA.ORCID 0000-0002-1867-8763

Funding

Request to Transfer R01GM105707-03 Novel regulatory mechanisms of Drosophila Pumilio and NanosR01GM105707 · NIGMS · UNIVERSITY OF MINNESOTA · PI GOLDSTROHM, AARON CHARLES · 2013 to 2022
$2.7M
mRNA regulatory functions of the Drosophila TRIM-NHL protein, BratR01GM145835 · NIGMS · UNIVERSITY OF MINNESOTA · PI GOLDSTROHM, AARON CHARLES · 2022 to 2025
$1.4M
NIGMS NIH HHS R01 GM105707NIGMS NIH HHS R01 GM145835
6 · The paper itself

Abstract

Brain tumor (Brat) is a Drosophila TRIM-NHL protein required for embryogenesis and neural stem cell differentiation. Although structural and biochemical studies established that the Brat NHL domain specifically binds RNA, the in vivo requirement for this activity has not been directly tested. Here, we used structure-guided mutagenesis and genome engineering to determine whether RNA recognition is essential for Brat function during development. The direct interaction between Brat's NHL domain and RNA containing Brat Binding Sites (BBS) can be abolished by alanine substitution of three separate residues on the NHL surface. We introduced these point mutations into the endogenous

Indexed as

CRISPRdevelopmentembryogenesisneural stem cellsRNA-binding protein

Identifiers

PMID42124648
PMCPMC13160073

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.