Antibiotic-Free Expression of γ-Glutamyl Transpeptidase in

Ran Zhang, Bo Jiang, Ziqun Xiao, Longbei Xiang

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ran ZhangSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Bo JiangSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, China.ORCID 0000-0002-0638-6456

Ziqun XiaoSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Longbei XiangSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Funding

National Key R&D Program of China 2022YFC2104900

6 · The paper itself

Abstract

L-theanine is a characteristic non-proteinogenic amino acid found in tea leaves and has attracted considerable attention because of its diverse physiological activity and broad application prospects. γ-glutamyl transpeptidase (GGT) can catalyze the synthesis of L-theanine from L-glutamine and ethylamine without ATP consumption, highlighting its advantages for enzymatic production. In this study, a complete process was established for L-theanine production. Through screening of single and dual promoters, the optimal expression combination, PyxiE-PspoVG, was identified. Furthermore, by integrating the

Indexed as

Bacillus subtilisgamma-GlutamyltransferaseGlutamatesEthylaminesFermentationGlutaminePromoter Regions, GeneticethylamineEthylaminesgamma-GlutamyltransferaseGlutamatesGlutaminetheanineantibiotic-free expressionBacillus subtilisL-theanineseparation and purificationγ-glutamyl transpeptidase

Identifiers

PMID42123842

DOI10.3390/molecules31091476

PMCPMC13165169

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.