Development and Validation of an LC-MS/MS Method for the Quantitation of JNJ-64619178 (JNJ) in Mouse Plasma: Characterization of In Vitro and In Vivo Pharmacokinetic Properties.

Nusrat Ahmed, Pratiksha Kshirsagar, Ling Ding, Daryl J Murry, Nagendra K Chaturvedi, Yashpal S Chhonker

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Nusrat AhmedClinical Pharmacology Laboratory, Department of Pharmacy Practice and Science, University of Nebraska Medical Center, Omaha, NE 68198, USA.ORCID 0000-0002-6059-0247

Pratiksha KshirsagarClinical Pharmacology Laboratory, Department of Pharmacy Practice and Science, University of Nebraska Medical Center, Omaha, NE 68198, USA.ORCID 0000-0001-9936-7125

Ling DingClinical Pharmacology Laboratory, Department of Pharmacy Practice and Science, University of Nebraska Medical Center, Omaha, NE 68198, USA.ORCID 0000-0002-4129-4418

Daryl J MurryClinical Pharmacology Laboratory, Department of Pharmacy Practice and Science, University of Nebraska Medical Center, Omaha, NE 68198, USA.ORCID 0000-0002-4169-5027

Nagendra K ChaturvediDivision of Hematology/Oncology, Department of Pediatrics, University of Nebraska Medical Center, Omaha, NE 68198, USA.ORCID 0000-0001-7037-898X

Yashpal S ChhonkerClinical Pharmacology Laboratory, Department of Pharmacy Practice and Science, University of Nebraska Medical Center, Omaha, NE 68198, USA.

Funding

Team Jack Foundation Power 5 GrantUNMC CHRI-PCRG LB905

6 · The paper itself

Abstract

Overexpression of protein arginine methyltransferase 5 (PRMT5) is pivotal in MYC-driven primary medulloblastoma tumors, suggesting PRMT5 as a potential therapeutic target. JNJ, a potent PRMT5 inhibitor currently in clinical trials, notably for non-Hodgkin lymphoma and lung cancer, was evaluated in this study. We report a validated LC-MS/MS bioanalytical method for quantifying JNJ in plasma and tissue matrices. The method demonstrated acceptable sensitivity, selectivity, and robustness in accordance with regulatory guidelines. The assay was linear over the range 0.2-500 ng mL

Indexed as

Liquid Chromatography-Mass SpectrometryTandem Mass SpectrometryAnimalsCaco-2 CellsChromatography, LiquidHumansMedulloblastomaMiceMicrosomes, LiverProtein-Arginine N-MethyltransferasesReproducibility of ResultsProtein-Arginine N-MethyltransferasesLC-MS/MS bioanalysismedulloblastomapharmacokineticsvalidation

Identifiers

PMID42123761

DOI10.3390/molecules31091396

PMCPMC13164762

What OpenQuestion holds

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.