Evidence map›Paper›PMID 42123326›Full record

ArticleInternational journal of molecular sciences2026

Metagenomic Profiling Reveals Extensive Bacterial Diversity in Chicken Manure and Associated Contaminated Wastewater.

Sadir Zaman, Nawab Ali, Waheed Ullah, Nadia Taimur, Noor Ul Akbar, Aiman Waheed, Niaz Muhammad, Muhammad Saeed Khan

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sadir ZamanDepartment of Microbiology, Kohat University of Science and Technology, Kohat 26000, Pakistan.ORCID 0000-0003-2325-2158
Nawab AliCollege of Engineering and Energy, Abdullah Al-Salem University, Khaldiya Campus, Kuwait City 72300, Kuwait.
Waheed UllahDepartment of Microbiology, Kohat University of Science and Technology, Kohat 26000, Pakistan.
Nadia TaimurDepartment of Botany, Kohat University of Science and Technology, Kohat 26000, Pakistan.
Noor Ul AkbarDepartment of Zoology, Kohat University of Science and Technology, Kohat 26000, Pakistan.
Aiman WaheedCentre of Biotechnology and Microbiology, University of Peshawar, Peshawar 25120, Pakistan.ORCID 0009-0009-6469-1377
Niaz MuhammadDepartment of Microbiology, Kohat University of Science and Technology, Kohat 26000, Pakistan.ORCID 0009-0000-7888-7754
Muhammad Saeed KhanDepartment of Microbiology, Kohat University of Science and Technology, Kohat 26000, Pakistan.ORCID 0009-0009-2563-6236

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chicken manure and its potential to contaminate water systems through the dispersal of pathogenic bacteria are major concerns in environmental and public health. In this study, a metagenomic analysis was employed to systematically identify and compare bacterial assemblages in chicken manure (CM) and in a contaminated sample of chicken manure wastewater (CMW). Whole DNA was extracted from CM and CMW, followed by whole-genome shotgun sequencing; data analysis was done using online Galaxy software (ver. 26.0.1.dev1). Metagenomic analysis reveals a complex One Health challenge. Data showed that CM and CMW are different in their microbiota, as indicated by a distinct separation of beta diversity values and limited overlapping of species between sample types. In the current study, we found a greatly significant common functional set of adapted bacterial masses, including major pathogenic bacterial groups as well as opportunistic and environmental bacterial species, indicative of a direct contamination from CM and CMW. Notably, in both CM and CMW, a plethora of opportunistic, enteric, and environmental pathogens like

Indexed as

BacteriaManureMetagenomeMetagenomicsWastewaterAnimalsBiodiversityChickensMicrobiotaManureWastewaterchicken manuremetagenomicspathogen disseminationwastewater microbiome

Identifiers

PMID42123326
PMCPMC13163441

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.