Evidence map›Paper›PMID 42121896›Full record

ArticleCells2026

Mass Cytometry Reveals Comparable Proportions of Leukocyte Subsets and Cell Surface P2X7 or CD39 on Human Peripheral Blood Mononuclear Cell Samples Isolated by Ficoll-Paque or SepMate Tube Density Gradient Centrifugation.

Amal Elhage, Bavani Gunasegaran, Natalie Smith, Ross J Turner, Debbie Watson, Helen M McGuire, Ronald Sluyter

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Amal ElhageMolecular Horizons and School of Science, University of Wollongong, Wollongong, NSW 2522, Australia.
Bavani GunasegaranCharles Perkins Centre, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.ORCID 0000-0002-4783-8404
Natalie SmithCharles Perkins Centre, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.
Ross J TurnerMolecular Horizons and School of Science, University of Wollongong, Wollongong, NSW 2522, Australia.ORCID 0000-0003-3762-6296
Debbie WatsonMolecular Horizons and School of Science, University of Wollongong, Wollongong, NSW 2522, Australia.
Helen M McGuireCharles Perkins Centre, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.ORCID 0000-0003-2047-6543
Ronald SluyterMolecular Horizons and School of Science, University of Wollongong, Wollongong, NSW 2522, Australia.ORCID 0000-0003-4909-686X

Funding

University of Wollongong N/A
6 · The paper itself

Abstract

P2X7 is an adenosine 5'-triphosphate (ATP)-gated ion channel. CD39 (ectonucleotidase triphosphate diphosphohydrolase-1) hydrolyses ATP to reduce its extracellular concentration to limit P2X7 activation. Both P2X7 and CD39 are present on leukocytes. Ficoll-Paque density gradient centrifugation is widely used to isolate human peripheral blood mononuclear cells (PBMCs), yet the extent to which other density gradient centrifugation methods influence the expression of cell surface molecules remains unclear. Using mass cytometry, this study compared the proportions of mononuclear leukocyte subsets and the relative amount of cell surface P2X7 and CD39 detected on these cells in paired, bar-coded cryopreserved human PBMC samples isolated by Ficoll-Paque or SepMate Tube density gradient centrifugation. Both techniques yielded similar proportions of mononuclear leukocyte subsets. P2X7 and CD39 were detected across all cell subsets, with the relative amount of P2X7 or CD39 comparable between separation methods. Relatively minor but statistically significant differences were observed for some populations. P2X7 expression was higher on CD3

Indexed as

Antigens, CDApyraseFlow CytometryLeukocytes, MononuclearReceptors, Purinergic P2X7Cell SeparationCentrifugation, Density GradientFicollHumansAntigens, CDApyraseCD39 antigenENTPD1 protein, humanFicollReceptors, Purinergic P2X7cell separationdendritic cellENTPD1lymphocytemass cytometrymonocyteNTPDase1P2RX7

Identifiers

PMID42121896
PMCPMC13162788

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.