ArticleAnimals : an open access journal from MDPI2026
Evaluation and Selection of Stable Reference Genes for qRT-PCR Analysis in Different Tissues of
Zhongdian Dong, Jiahao Gao, Xiaobin Li, Zhishan Chen, Jianjun Li, Jian Liao, Yanping Zhang, Zhongduo Wang, Yusong Guo, Ning Zhang
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In one paragraphArticle in Animals : an open access journal from MDPI, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
10 authors.
Zhongdian DongKey Laboratory of Aquaculture in the South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.ORCID 0000-0001-7898-5223 Jiahao GaoKey Laboratory of Aquaculture in the South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.
Xiaobin LiKey Laboratory of Aquaculture in the South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.
Zhishan ChenKey Laboratory of Aquaculture in the South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.
Jianjun LiGuangdong Provincial Laboratory Animals Monitoring Center, Guangdong Provincial Biotechnology Research Institute, Guangzhou 510663, China.
Jian LiaoKey Laboratory of Aquaculture in the South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.ORCID 0009-0005-0459-2060 Yanping ZhangKey Laboratory of Aquaculture in the South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.ORCID 0000-0002-7142-4121 Zhongduo WangKey Laboratory of Aquaculture in the South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.
Yusong GuoKey Laboratory of Aquaculture in the South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.
Ning ZhangKey Laboratory of Aquaculture in the South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.ORCID 0000-0003-2845-4787 Funding
No grant is acknowledged in the PubMed record.
6 · The paper itselfAbstract
Reliable normalization is essential for accurate quantitative real-time PCR (qRT-PCR) analysis, yet suitable reference genes have not been systematically evaluated in
Indexed as
environmental contaminantsgene expression normalizationMugilogobius chulaereference genetissue-specific stability
Identifiers
PMID42121831
PMCPMC13163046
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