Evidence map›Paper›PMID 42121449›Full record

ArticleFoods (Basel, Switzerland)2026

A Cyanide-Free UHPLC-MS/MS Workflow for the Analysis of Major Cobalamin Vitamers in Foods.

Fengen Wang, Li Cao, Min Ding, Ruiju Li, Chao Zhang, Baorui Li, Zhaowei Yang, Kaizhen Liu, Jiamei Xin, Xia Li and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Foods (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Fengen WangInstitute of Agricultural Products Quality Safety and Standards, Shandong Academy of Agricultural Sciences, Jinan 250100, China.ORCID 0000-0002-7236-4669
Li CaoInstitute of Agricultural Products Quality Safety and Standards, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Min DingInstitute of Agricultural Products Quality Safety and Standards, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Ruiju LiInstitute of Agricultural Products Quality Safety and Standards, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Chao ZhangInstitute of Agricultural Products Quality Safety and Standards, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Baorui LiInstitute of Food and Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Zhaowei YangQinhuangdao Agriculture Product Quality Safety Inspection and Monitoring Center, Qinhuangdao Municipal Bureau of Agriculture and Rural Affairs, Qinhuangdao 066099, China.
Kaizhen LiuCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Jiamei XinInstitute of Food and Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Xia LiInstitute of Agricultural Products Quality Safety and Standards, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Tongcheng XuInstitute of Food and Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Ligang DengInstitute of Agricultural Products Quality Safety and Standards, Shandong Academy of Agricultural Sciences, Jinan 250100, China.

Funding

Ministry of Science and Technology 2022YFF1100603Ministry of Science and Technology YDZX2024146Shandong Academy of Agricultural Sciences 2023CZ01
6 · The paper itself

Abstract

Accurate determination of cobalamin vitamers in foods remains analytically challenging because conventional cyanidation-based methods convert native cobalamins into cyanocobalamin (CNCbl) and may distort their original distribution. In this study, a cyanide-free UHPLC-MS/MS workflow was developed for the analysis of major cobalamin vitamers in foods, with particular emphasis on preserving native forms during sample preparation. Light, temperature, and cleanup procedures were systematically evaluated. Methylcobalamin (MeCbl) and adenosylcobalamin (AdoCbl) showed pronounced light sensitivity, whereas red-light handling better preserved vitamer integrity during pre-analytical operations. A tandem cleanup procedure combining immunoaffinity and Oasis HLB solid-phase extraction improved extract cleanliness in complex food matrices. The workflow showed good chromatographic separation and excellent linearity (R

Indexed as

cobalamin vitamerscyanide-free analysisfood matriceslight stabilitytandem cleanupUHPLC-MS/MS

Identifiers

PMID42121449
PMCPMC13163423

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.