Evidence map›Paper›PMID 42120803›Full record

ArticleAnnals of biomedical engineering2026

Click Chemistry-Based Quantification of Extracellular Matrix Turnover for Drug Screening and Regenerative Medicine.

Annie Porter, Songshan Fan, Ying Peng, Mengxi Lv, Yilu Zhou, Abdulaziz Alanazi, Lin Han, Liyun Wang, X Lucas Lu

Abstract read
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Article in Annals of biomedical engineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Nascent Extracellular Matrix Converts Biomaterial Cues into Cell Fate Decisions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  2. Article
  3. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Annie PorterDepartment of Mechanical Engineering, University of Delaware, 130 Academy Street, Newark, DE, 19716, USA.ORCID http://orcid.org/0000-0002-8207-0357
Songshan FanDepartment of Mechanical Engineering, University of Delaware, 130 Academy Street, Newark, DE, 19716, USA.
Ying PengDepartment of Mechanical Engineering, University of Delaware, 130 Academy Street, Newark, DE, 19716, USA.
Mengxi LvDepartment of Mechanical Engineering, University of Delaware, 130 Academy Street, Newark, DE, 19716, USA.
Yilu ZhouDepartment of Mechanical Engineering, University of Delaware, 130 Academy Street, Newark, DE, 19716, USA.
Abdulaziz AlanaziSchool of Biomedical Engineering, Science and Health Systems, Drexel University, 3141 Chestnut St, Philadelphia, PA, 19104, USA.
Lin HanSchool of Biomedical Engineering, Science and Health Systems, Drexel University, 3141 Chestnut St, Philadelphia, PA, 19104, USA.
Liyun WangDepartment of Mechanical Engineering, University of Delaware, 130 Academy Street, Newark, DE, 19716, USA.
X Lucas LuDepartment of Mechanical Engineering, University of Delaware, 130 Academy Street, Newark, DE, 19716, USA. xlu@udel.edu.ORCID http://orcid.org/0000-0001-6905-6887

Funding

Understanding synovial macrophage inflamm-aging within osteoarthritisP20GM139760 · NIGMS · UNIVERSITY OF DELAWARE · PI DAWN M ELLIOTT · 2021 to 2026
$19.1M
Biomechanics of Cartilage: Roles of Decorin in ECM Assembly and DegradationR01AR074490 · NIAMS · DREXEL UNIVERSITY · PI Lin Han · 2019 to 2026
$2.3M
Prevention of PTOA via regulation of the cytomechanics of chondrocytesR01AR074472 · NIAMS · UNIVERSITY OF DELAWARE · PI LU, X. LUCAS · 2020 to 2025
$1.5M
Division of Graduate Education Graduate Research Fellowship ProgramNational Science Foundation CMMI-1751898NIAMS NIH HHS R01 AR074472NIAMS NIH HHS R01 AR074490NIGMS NIH HHS P20 GM139760NIH HHS P20GM139760NIH HHS R01AR074472NIH HHS R01AR074490University of Delaware Helwig Fellowship in Mechanical EngineeringU.S. Department of Defense W81XWH-13-1-0148
6 · The paper itself

Abstract

purposeTo develop and validate a bioorthogonal labeling approach for quantifying ECM remodeling in living cell and tissue culture systems.

methodsStrain-promoted azide-alkyne (SPAAC) reactions, or copper-free click chemistry, were used to fluorescently label newly synthesized glycan and protein matrix components. ECM synthesis and degradation was quantified in cartilage explants, human mesenchymal stem cells, and SKBR3 breast cancer cells under various external stimuli, including inflammation, mechanical stimulus, and drug treatment.

resultsThe click chemistry method reliable quantified ECM turnover across platforms. It detected reduced glycan and protein synthesis after 24-hour inflammatory challenge and enabled longitudinal tracking of ECM degradation in cartilage explants. The technique demonstrated high sensitivity, measuring increased ECM deposition by ~ 10,000 human mesenchymal stem cells in 12-hour intervals and substrate stiffness-dependent synthesis by SKBR3 cells. Additionally, the approach supported osteoarthritis drug screening by identifying compounds that mitigated inflammation-induced ECM degradation.

conclusionCompared to traditional biochemical or histological assays, the click chemistry-based technique provides higher sensitivity, reduced sample requirements, and improved temporal resolution for quantifying ECM turnover. Its versatility enables broad application in tissue engineering, regenerative medicine, disease modeling, and high-throughput drug evaluation.

Indexed as

CartilageGlycanMetabolic labelingProteinTissue engineering

Identifiers

PMID42120803

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.