ArticlePlant molecular biology2026
Development of efficient Agrobacterium-mediated transformation of a second-generation biofuel crop, Brassica carinata.
Article in Plant molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Metabolic engineering of cereal lipids: from omega-3 fatty acids to wax esters and pheromones.Advanced biotechnology · 2026Review
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6 authors.
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Abstract
Brassica carinata (B. carinata) is a promising non-food biofuel crop with the potential for low carbon intensity biofuel production under suitable management conditions. However, B. carinata requires extensive genetic improvement of many agronomic traits for it to be widely deployed into agriculture systems. Its rapid improvement via gene editing or genetic modification requires robust, genotype-flexible transformation protocols, as current methods are limited and have only been applied to a narrow germplasm base, hindering broader genetic enhancement efforts. In this paper, cotyledonary petioles from five diverse B. carinata genotypes were evaluated for shoot regeneration under different cytokinin treatments. Among the genotypes tested, regeneration efficiency varied substantially across the five B. carinata genotypes. Transient expression of the GUS reporter gene in cotyledonary petioles confirmed high transformation proficiency, with expression frequencies varying from 96.4 to 100% among four genotypes. Consistently, high stable transformation efficiency was achieved for these four genotypes of at least 5%, with the highest efficiency being 12%. Interestingly, transformation and regeneration efficiencies did not correlate, indicating the latter does not serve as a reliable indicator of transformation efficiency when subjected to selection pressures. Transgene integration was validated by PCR and copy number analysis was performed using digital droplet PCR. Furthermore, T
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