Evidence map›Paper›PMID 42119981›Full record

ArticleThe Journal of biological chemistry2026

INAAC: An affinity chromatography strategy enabling characterization and quantification of influenza neuraminidase antigens in vaccines.

Hyeog Kang, Anna M Borowska, Tapan Kanai, Jin Gao, Hai Yu, Xi Chen, Jason Gorman, Dirk-Jan Slotboom, Robert Daniels

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hyeog KangDivision of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
Anna M BorowskaFaculty of Science and Engineering, Groningen Biomolecular Sciences and Biotechnology, Membrane Enzymology Group, University of Groningen, Groningen, the Netherlands.
Tapan KanaiDivision of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
Jin GaoDivision of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
Hai YuDepartment of Chemistry, University of California, Davis, California, USA.
Xi ChenDepartment of Chemistry, University of California, Davis, California, USA.
Jason GormanDivision of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
Dirk-Jan SlotboomFaculty of Science and Engineering, Groningen Biomolecular Sciences and Biotechnology, Membrane Enzymology Group, University of Groningen, Groningen, the Netherlands.
Robert DanielsDivision of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA. Electronic address: robert.daniels@fda.hhs.gov.

Funding

Affordable sialoglycans and associated reagents for expanded chemoenzymatic productionR42GM143998 · NIGMS · INTEGRATED MICRO-CHROMATOGRAPHY SYSTEMS, INC. · PI LEE, LIM ANDREW · 2021 to 2024
$2.6M
NIGMS NIH HHS R42 GM143998
6 · The paper itself

Abstract

Seasonal influenza vaccines are commonly produced using viruses containing hemagglutinin (HA) and neuraminidase (NA) antigens from recommended strains. However, NA amounts are not monitored due to lack of strategies for isolating NA reference antigens from viruses, limiting the ability to evaluate any contributions from NA for more than 50 years. Here, we developed an influenza neuraminidase active-site affinity chromatography (INAAC) strategy that uses an active-site binding antibody to isolate functional NAs from influenza A and B vaccine strains. INAAC recovered 20 to 60% of detergent-solubilized NA activity from vaccine viruses and recombinant sources, with CaCl

Indexed as

Antigens, ViralChromatography, AffinityInfluenza VaccinesNeuraminidaseAnimalsCatalytic DomainInfluenza A Virus, H1N1 SubtypeInfluenza B virusAntigens, ViralInfluenza VaccinesNeuraminidaseenveloped virus surface antigensINAACinfluenza NA active-site affinity chromatographyinfluenza surface antigensinfluenza virus vaccinesNA purificationNA vaccine standardsstructure of native NA vaccine antigenszanamivir

Identifiers

PMID42119981
PMCPMC13264171

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.