Evidence map›Paper›PMID 42119891›Full record

ArticleVirus research2026

Characterization and antibacterial efficacy of the broad-host-range phage P108 against biofilm-forming and methicillin-resistant Staphylococcus aureus.

Xuemei Wei, Ruiyang Zhang, Jianglin Liao, He Liu, Zhen Hu, Weilong Shang, Jing Wang, Ming Li, Xiancai Rao, Shuguang Lu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virus research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xuemei WeiMedical Research Institute, Southwest University, Chongqing 400716, China. Electronic address: 1310622412@qq.com.
Ruiyang ZhangDepartment of Microbiology, College of Basic Medical Sciences, Army Medical University, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Chongqing 400038, China. Electronic address: zhangruiyang@tmmu.edu.cn.
Jianglin LiaoDepartment of Cardiovascular Medicine, Center for Circadian Metabolism and Cardiovascular Disease, Southwest Hospital, Army Medical University, Chongqing 400038, China. Electronic address: 1209062269@qq.com.
He LiuDepartment of Cardiology, Xinqiao Hospital, Army Medical University, Chongqing 400037, China. Electronic address: 2216774903@qq.com.
Zhen HuDepartment of Microbiology, College of Basic Medical Sciences, Army Medical University, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Chongqing 400038, China. Electronic address: huzhenzhen1314@tmmu.edu.cn.
Weilong ShangDepartment of Microbiology, College of Basic Medical Sciences, Army Medical University, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Chongqing 400038, China. Electronic address: shangwl@tmmu.edu.cn.
Jing WangDepartment of Microbiology, College of Basic Medical Sciences, Army Medical University, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Chongqing 400038, China. Electronic address: wangjing2008@tmmu.edu.cn.
Ming LiDepartment of Microbiology, College of Basic Medical Sciences, Army Medical University, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Chongqing 400038, China. Electronic address: liming@tmmu.edu.cn.
Xiancai RaoMedical Research Institute, Southwest University, Chongqing 400716, China; Department of Microbiology, College of Basic Medical Sciences, Army Medical University, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Chongqing 400038, China. Electronic address: xcrao@tmmu.edu.cn.
Shuguang LuDepartment of Microbiology, College of Basic Medical Sciences, Army Medical University, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Chongqing 400038, China. Electronic address: lusg@tmmu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Staphylococcus aureus (S. aureus) is a widely distributed opportunistic pathogen capable of causing a variety of serious infections, with its antibiotic resistance becoming increasingly prevalent. As natural bactericidal agents, bacteriophages (phages) have emerged as promising therapeutic alternatives to antibiotics. In this study, we isolated a lytic phage P108 capable of targeting methicillin-resistant S. aureus (MRSA) strains. Phage P108 features an icosahedral head with a diameter of approximately 84.7 nm and a contractile tail measuring about 221.5 nm in length. It has a latent period of 20 min and completes lysis within 60 min. Whole-genome sequencing revealed a linear dsDNA genome of 140,807 bp, encoding 226 putative proteins and 3 tRNAs. Four novel structural protein-coding genes were identified. Phylogenetic analysis demonstrated that P108 represents a member of the Herelleviridae family, Kayvirus genus. P108 is capable of lysing 79.2% (95/120) of clinical isolates of S. aureus and demonstrates broad-spectrum lytic activity against MRSA (84.8%, 39/46). Furthermore, phage P108 exhibits high stability, potent in vitro bactericidal activity, and effective bacterial biofilm removal, outperforming vancomycin in overall efficacy. These findings highlight its potential for antibacterial applications and support its development as a novel therapeutic strategy against drug-resistant S. aureus infections.

Indexed as

BiofilmsHost SpecificityMethicillin-Resistant Staphylococcus aureusStaphylococcus PhagesAnti-Bacterial AgentsBacteriolysisDNA, ViralGenome, ViralHumansPhylogenyStaphylococcal InfectionsWhole Genome SequencingAnti-Bacterial AgentsDNA, ViralAntibacterial efficacyAntibiofilm activityBroad host rangeGenomic analysisMethicillin-resistant Staphylococcus aureus (MRSA)Phage P108

Identifiers

PMID42119891
PMCPMC13200134

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.