ArticleCell reports methods2026
HyPIC-3D enables characterization of migratory cancer cell subpopulations in 3D hypoxic microenvironments.
Article in Cell reports methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Malignant tumors harbor cancer cell subpopulations with heterogeneous migratory behaviors. Identifying the molecular programs specifying whether these subpopulations migrate or remain stationary is essential for preventing metastasis and requires experimental approaches that directly separate and analyze these subpopulations as they move throughout 3D microenvironments. Here, we present "paired isolation and characterization in 3D hypoxic microenvironments" (HyPIC-3D), a method to isolate and characterize migratory and non-migratory cancer cell subpopulations from the same pool of spheroids. By adapting tissue culture inserts used for migration assays, we established a migration platform that preserves oxygen gradients and the spheroid architecture. HyPIC-3D shows that the pattern and extent of migration differ among cancer cell types while identifying distinct molecular switches and oxygen requirements underlying this heterogeneity. Integrated with image analysis tools, HyPIC-3D is implemented with standard computational and laboratory equipment and is amenable to diverse downstream applications, enabling mechanistic dissection of migration and discovery of metastatic regulators.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.