ArticleSTAR protocols2026
Protocol to assess intracellular labile iron and oxygen at single-cell resolution using a genetically encoded ratiometric reporter.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Abstract
Here, we present a protocol to acquire, visualize, and quantify intracellular labile iron and oxygen signals at single-cell resolution using LiON (labile iron and oxygen notifier), a genetically encoded ratiometric reporter. We describe steps for fluorescence microscopy and flow cytometry acquisition, ratio-map visualization, and region of interest (ROI)-based quantification. We then detail procedures for single-cell time-lapse tracing. For complete details on the use and execution of this protocol, please refer to Maeda et al.
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