Evidence map›Paper›PMID 42118660›Full record

ArticleSTAR protocols2026

Protocol for fibrin-based endothelial-cardiomyocyte co-culture in a microfluidic device.

Talitha C F Spanjersberg, Aram Klaassen, Richard Wubbolts, Christian G M van Dijk, Jolanda van der Velden, Rogier J A Veltrop, Pim van der Harst, Magdalena Harakalova, Frank G van Steenbeek

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Talitha C F SpanjersbergRegenerative Medicine Center Utrecht (RMCU), University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands; Division Heart & Lungs, Department of Cardiology, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands; Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.
Aram KlaassenRegenerative Medicine Center Utrecht (RMCU), University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands; Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.
Richard WubboltsRegenerative Medicine Center Utrecht (RMCU), University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands; Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.
Christian G M van DijkRegenerative Medicine Center Utrecht (RMCU), University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands; Department of Nephrology and Hypertension, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands.
Jolanda van der VeldenDepartment of Physiology, Amsterdam UMC, Location VUmc, Amsterdam, the Netherlands.
Rogier J A VeltropRegenerative Medicine Center Utrecht (RMCU), University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands; Division Heart & Lungs, Department of Cardiology, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands; Cardiovascular Research Institute Maastricht (CARIM), Maastricht, the Netherlands.
Pim van der HarstRegenerative Medicine Center Utrecht (RMCU), University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands.
Magdalena HarakalovaRegenerative Medicine Center Utrecht (RMCU), University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands; Division Heart & Lungs, Department of Cardiology, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands; Cardiovascular Research Institute Maastricht (CARIM), Maastricht, the Netherlands.
Frank G van SteenbeekRegenerative Medicine Center Utrecht (RMCU), University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands; Division Heart & Lungs, Department of Cardiology, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands; Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands. Electronic address: f.g.vansteenbeek@uu.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol for building a vascularized heart-on-a-chip by co-encapsulating human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes and endothelial cells in vascular endothelial growth factor (VEGF)- and aprotinin-supplemented fibrin. We describe steps for cell preparation, fibrin formulation, chip loading, optional droplet-based pilot or high-throughput setups, and imaging. We then detail procedures for immunofluorescence staining and routine quantitative analysis of vascular and contractile features during culture for up to three weeks.

Indexed as

Endothelial CellsFibrinLab-On-A-Chip DevicesMyocytes, CardiacCoculture TechniquesHumansInduced Pluripotent Stem CellsVascular Endothelial Growth Factor AFibrinVascular Endothelial Growth Factor ACell-based AssaysCell cultureTissue Engineering

Identifiers

PMID42118660
PMCPMC13195612

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.