Evidence map›Paper›PMID 42118572›Full record

ArticleBiochemistry2026

A Miniaturized, High-Throughput Aqueous Solvent-Centric Method for Protein Solubility Screening.

Adrian Svoboda, Marina Molineris, Theodora Tureckiova, Klára Hlouchová, Tomáš Pluskal, Teo Hebra

Abstract read
In one paragraph

Article in Biochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Adrian SvobodaInstitute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo náměstí 542/2, 160 00 Prague, Czech Republic.
Marina MolinerisInstitute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo náměstí 542/2, 160 00 Prague, Czech Republic.
Theodora TureckiovaDepartment of Cell Biology, Faculty of Science, Charles University, Prague 12800, Czech Republic.
Klára HlouchováInstitute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo náměstí 542/2, 160 00 Prague, Czech Republic.ORCID 0000-0002-5651-4874
Tomáš PluskalInstitute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo náměstí 542/2, 160 00 Prague, Czech Republic.ORCID 0000-0002-6940-3006
Teo HebraInstitute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo náměstí 542/2, 160 00 Prague, Czech Republic.ORCID 0000-0002-5878-259X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Efficient access to soluble recombinant proteins remains a major bottleneck in biochemical and structural studies. We describe an aqueous, solvent-centric, fully miniaturized 96-well workflow to screen extraction conditions that preserve soluble recombinant protein during lysis and clarification in a single working day. Liquid-nitrogen-frozen

Indexed as

High-Throughput Screening AssaysRecombinant ProteinsEscherichia coliHydrogen-Ion ConcentrationMiniaturizationSolubilitySolventsWaterRecombinant ProteinsSolventsWaterBufferCryomillingHeterologous expressionProtein SolubilityRecombinant Protein

Identifiers

PMID42118572
PMCPMC13235557

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.