Tailoring ω-3 fatty acid enrichment through genipin, glutaraldehyde, and glyoxyl linked immobilization of Rhizomucor miehei lipase on MWCNTs.

Deniz Yildirim, Ahmet Tülek, Nurettin Paçal, N Ece Varan Faki, Ali Toprak, Dilek Alagöz, Ramazan Bilgin

Abstract read

In one paragraph

Article in World journal of microbiology & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Deniz YildirimFaculty of Engineering, Department of Chemical Engineering, Cukurova University, Adana, 01330, Türkiye. dyildirim@cu.edu.tr.ORCID http://orcid.org/0000-0002-5041-8160

Ahmet TülekFaculty of Engineering and Natural Sciences, Department of Molecular Biology and Genetics, Kütahya Health Sciences University, Merkez, Kütahya, 43100, Türkiye. ahmet.tulek@ksbu.edu.tr.ORCID http://orcid.org/0000-0003-1079-7837

Nurettin PaçalDepartment of Biology, Iğdır University, Postgraduate Education Institute, Iğdır, 76000, Türkiye.ORCID http://orcid.org/0009-0004-6491-0406

N Ece Varan FakiFaculty of Science and Letters, Department of Chemistry, Cukurova University, Adana, 01330, Türkiye.ORCID http://orcid.org/0000-0003-1570-9509

Ali ToprakAcigol Vocational School, Nevsehir Haci Bektas Veli University, Acigol, Nevsehir, 50140, Türkiye.ORCID http://orcid.org/0000-0002-6520-3226

Dilek AlagözImamoglu Vocational School, Cukurova University, Imamoglu, 01700, Adana, Türkiye.ORCID http://orcid.org/0000-0001-6761-6385

Ramazan BilginFaculty of Science and Letters, Department of Chemistry, Cukurova University, Adana, 01330, Türkiye.ORCID http://orcid.org/0000-0002-3592-8824

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Efficient enrichment of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) from natural sources remains a major challenge for sustainable ω-3 fatty acid production. This study reports the immobilization of Rhizomucor miehei lipase (RML) on multi-walled carbon nanotubes (MWCNTs) using three distinct coupling chemistries, including genipin (MWCNT/Gen@RML), glutaraldehyde (MWCNT/Glu@RML), and glyoxyl (MWCNT/Gly@RML). The resulting nanostructured biocatalysts were systematically evaluated for the selective enrichment of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) from commercial fish oil. The immobilized RML derivatives were characterized using FTIR, SEM, SEM-EDS, and TGA analysis. The maximum immobilized protein amounts were approximately 8.4, 8.1, and 8.6 mg g⁻

Indexed as

Enzymes, ImmobilizedFatty Acids, Omega-3LipaseNanotubes, CarbonRhizomucorBiocatalysisDocosahexaenoic AcidsEicosapentaenoic AcidFish OilsGlutaralGlyoxylatesHydrogen-Ion ConcentrationIridoidsTemperatureDocosahexaenoic AcidsEicosapentaenoic AcidEnzymes, ImmobilizedFatty Acids, Omega-3Fish OilsgenipinGlutaralGlyoxylatesIridoidsLipaseNanotubes, CarbonEPA and DHAFish oilMulti-walled carbon nanotubesRhizomucor miehei lipase

Identifiers

PMID42118478

DOI10.1007/s11274-026-05011-y

PMCPMC13167907

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.