Insights into the functions of MYB29 and MYB28.1 involved in glucosinolate biosynthesis from Isatis indigotica Fort.

Yan Zhang, Zhaojin Xu, Kaiwei Qin, Jiamin Kang, Limi Tang, Bowen Duan, Zhihua Liu, Tao Li

Abstract read

In one paragraph

Article in Functional & integrative genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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8 authors.

Yan ZhangNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China.

Zhaojin XuNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China.

Kaiwei QinNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China.

Jiamin KangNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China.

Limi TangNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China.

Bowen DuanNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China.

Zhihua LiuNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China.

Tao LiNational Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, 710119, China. litao@snnu.edu.cn.

Funding

Natural Science Foundation of Shaanxi Province 2023-JC-ZD-59

6 · The paper itself

Abstract

Glucosinolates (GSLs) have garnered substantial attention for their essential functions in bolstering plant defense responses and potential anti-cancer effects. It was reported that MYB29 and MYB28 contribute to the accumulation of aliphatic GSLs in Arabidopsis thaliana, yet their roles in Isatis indigotica Fort. remain elusive. In this study, IiMYB29 and IiMYB28.1 were cloned, the expression patterns, subcellular localization and their functions in GSL biosynthesis were carried out. The results revealed that IiMYB29 exerted a stimulatory effect on plant growth and development, whereas IiMYB28.1 displays a negative regulatory influence. In GSL biosynthesis process, a comprehensive analysis identified 9 differentially expressed genes (DEGs) in the transgenic IiMYB29 plant, whereas 19 DEGs detected in the transgenic IiMYB28.1 plant. Furthermore, IiMYB29 induced a notable decline in GSL content, plummeting to a minimum level of 0.166 times of wild type. Conversely, IiMYB28.1 elicited a substantial increase in GSL content, peaking at a maximum of 2.264 times of wild type. The experimental results initially revealed the regulatory mechanisms of IiMYB29 and IiMYB28.1 on GSL synthesis, and laid the foundation for molecular breeding of I. indigotica.

Indexed as

GlucosinolatesIsatisPlant ProteinsTranscription FactorsArabidopsisGene Expression Regulation, PlantPlants, Genetically ModifiedGlucosinolatesPlant ProteinsTranscription FactorsglucosinolateIiMYB28.1IiMYB29Isatis indigotica Fort.R2R3-MYB

Identifiers

PMID42118386

DOI10.1007/s10142-026-01865-5

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