ArticleJournal of extracellular biology2026
Proteomic and Super-Resolution Analyses of Murine Brown Adipocyte-Derived Extracellular Vesicles.
Article in Journal of extracellular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
9 authors.
Funding
Abstract
Thermogenic brown adipocytes (BAs) are not only an energy sink but also regulate systemic metabolic health through secreted factors and extracellular vesicles (EVs). Here, we investigate the baseline profiles BA-EVs and how cold-induced thermogenesis alters the proteome of BA-EVs. We first established and validated transgenic mouse models expressing human CD63 (hCD63)-GFP for visualisation and isolation of EVs exclusively from adipocytes and BAs. We then used super-resolution microscopy to compare the subcellular localisation and maturation of EVs in adipocytes grown in culture or directly isolated from mice, revealing distinct features. We then used immunoprecipitation to enrich BA-EVs and employed mass spectrometry to define the EV-proteomes. We discovered that cold stimulation elevated secretion of mitochondrial and lysosomal proteins in BA-EVs. We validated the presence and activity of creatine kinase B (CKB) in BA-EVs. We finally showed that BA-EVs promote thermogenic gene expression in white adipocytes, but cold stimulation surprisingly blunted EV's effect in up-regulating
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