Evidence map›Paper›PMID 42116847›Full record

ArticleISME communications2026

Converting relative amplicon abundances to absolute abundances via flow cytometry: metagenomic validation and application to long ocean transects.

Nathan L R Williams, Qicheng Bei, Yubin Raut, Jed A Fuhrman

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In one paragraph

Article in ISME communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Nathan L R WilliamsDepartment of Biological Sciences-Marine and Environmental Biology, University of Southern California, Los Angeles, CA 90026, United States.
Qicheng BeiDepartment of Biological Sciences-Marine and Environmental Biology, University of Southern California, Los Angeles, CA 90026, United States.ORCID https://orcid.org/0000-0002-5712-5440
Yubin RautDepartment of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, United States.ORCID https://orcid.org/0000-0003-3841-7023
Jed A FuhrmanDepartment of Biological Sciences-Marine and Environmental Biology, University of Southern California, Los Angeles, CA 90026, United States.ORCID https://orcid.org/0000-0002-2361-1985

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

With microbes critical for ocean ecological and biogeochemical processes, we need to understand their abundance and diversity distributions. While traditional amplicon sequencing provides only relative abundance data, and the strongly preferred absolute abundances can be determined from samples spiked with internal standards, few oceanographic studies with absolute abundances exist. However, many have flow cytometry (FCM) data that should allow us to retrospectively "anchor" the relative abundances into absolute abundances. We tested this hypothesis with data from the 29th Atlantic Meridional Transect (AMT29) cruise where we had FCM of

Indexed as

absolute microbial abundanceAtlantic Meridional transect (AMT)flow cytometry; amplicon sequencing correctionGlobal rRNA Universal Metabarcoding of plankton (GRUMP)internal standard normalizationmarine microbial ecologyProchlorococcus and SynechococcusrecA and radA metagenomicsrRNA gene copy estimation

Identifiers

PMID42116847
PMCPMC13157830

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.