ArticleScientific reports2026
In vivo and ex vivo evaluation of the responses of rat Müller glia after injury.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Retinal ex vivo systems have been widely used to investigate diverse physiological and pathological processes in the retina. Although retinal ex vivo systems are generally considered to approximate in vivo environment of the retina, careful evaluation of how ex vivo conditions affect cellular function is necessary for accurate interpretation of such data. Here we analyzed the responses of retinal Müller glia to photoreceptor injury in vivo and ex vivo to assess the effects of ex vivo culture on the phenotypes of Müller glia in pathological contexts. A rat model of photoreceptor injury was generated by intraperitoneal injection of methyl methanesulfonate (MMS) and retinal ex vivo cultures were prepared 1 or 2 days after MMS treatment. A substantial number of Müller glia die by apoptosis after injury-induced proliferation in vivo, but not ex vivo. While the pattern of Müller glia proliferation was similar between the models, the extent of phagocytic activity, migration, DNA damage response, and neurogenic factor expression varied across the models. These findings reveal previously unrecognized effects of ex vivo culture on Müller glia in disease contexts, highlighting the need for caution when assessing injury-induced phenotypes of Müller glia using retinal ex vivo systems.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.