Evidence map›Paper›PMID 42113358›Full record

ArticleActa parasitologica2026

Optimization of Serum-Free Culture Medium for Theileria annulata-Transformed Cell Lines: A Comparative Analysis.

Peiqi Liu, Shuaiyang Zhao, Jiali Yao, Zeen Liu, Muhammad Kashif Obaid, Hong Yin, Guiquan Guan

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Acta parasitologica, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Peiqi LiuState Key Laboratory of Animal Disease Control and Prevention/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, 730046, Lanzhou, Gansu, China.
Shuaiyang ZhaoState Key Laboratory of Animal Disease Control and Prevention/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, 730046, Lanzhou, Gansu, China. zhaoshuaiyang@caas.cn.
Jiali YaoState Key Laboratory of Animal Disease Control and Prevention/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, 730046, Lanzhou, Gansu, China.
Zeen LiuState Key Laboratory of Animal Disease Control and Prevention/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, 730046, Lanzhou, Gansu, China.
Muhammad Kashif ObaidState Key Laboratory of Animal Disease Control and Prevention/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, 730046, Lanzhou, Gansu, China.
Hong YinState Key Laboratory of Animal Disease Control and Prevention/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, 730046, Lanzhou, Gansu, China. yinhong@caas.cn.
Guiquan GuanState Key Laboratory of Animal Disease Control and Prevention/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, 730046, Lanzhou, Gansu, China. guanguiquan@caas.cn.

Funding

Agricultural 444 Science and Technology Innovation Program (ASTIP) CAAS-ASTIP-2016-LVRINational Key 443 Research and Development Program of China 2024YFD1800104National Key Research and Development Program of China 2023YFD1802403National the Natural Science Foundation of China grant no. 442 32202836Natural Science Foundation of Gansu Province grant no. 2grant no. 24JRRA015NBCITS grant no. CARS-37
6 · The paper itself

Abstract

purposeCultivation of Theileria annulata schizont-transformed cell lines typically relies on fetal bovine serum (FBS), limiting standardization. This study evaluated three serum-free media (SFMs) for the in-vitro cultivation of T. annulata-transformed cell lines derived from different isolates.

methodsTaNM1 and TaXJS cell lines were adapted to AIM-V, VP-SFM, and HL-1, and compared against FBS-supplemented RPMI-1640. Proliferation, apoptosis, and parasite burden were assessed via viable cell counting, flow cytometry, and a novel TaSP-targeted absolute quantitative PCR (qPCR).

resultsAIM-V SFM and VP-SFM supported sustained proliferation of both cell lines after adaptation. AIM-V SFM showed the most stable performance among the tested serum-free conditions, with mean doubling times of 35.45 h for TaNM1 and 52.45 h for TaXJS. In contrast, unsupplemented HL-1 did not support robust growth, whereas supplementation with hypoxanthine-thymidine(HT) and AlbuMax I partially restored growth. Flow cytometry showed no marked increase in apoptosis under serum-free conditions, and qPCR indicated maintenance of T. annulata genome copy numbers during serum-free culture.

conclusionsSerum-free culture of T. annulata schizont-transformed cell lines is feasible but strongly medium-dependent. AIM-V SFM and VP-SFM supported serum-free maintenance after adaptation, whereas HL-1 required supplementation. These findings provide a basis for further optimization of serum-free culture systems for standardized research and vaccine-related applications.

Indexed as

Cell Culture TechniquesTheileria annulataAnimalsApoptosisCattleCell Line, TransformedCell ProliferationCulture Media, Serum-FreeFlow CytometryCulture Media, Serum-FreeAbsolute qPCRIn vitro cultureSchizont-transformed cell linesSerum-free mediaTheileria annulata

Identifiers

PMID42113358

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