Evidence map›Paper›PMID 42113344›Full record

ArticleWorld journal of microbiology & biotechnology2026

Formulation of Trichoderma spp. encapsulated in alginate: potential for biofungicide with controlled conidial release.

Thalesram Izidoro Pinotti, Vinicius de Paula Taffarel, Gabriel de Araújo Silva Cipriano, Ionnara Diogo Xavier, Yanka Manoelly Dos Santos Gaspar, Luysa Valéria Leal Coêlho Ramos, Augusto Matias de Oliveira, Marcos Antonio Barbosa de Lima, Galba Maria de Campos-Takaki, Tiago de Oliveira Sousa and 2 more

Abstract read
In one paragraph

Article in World journal of microbiology & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Thalesram Izidoro PinottiCampus Professora Cinobelina Elvas, Federal University of Piauí, Bom Jesus, 64900-000, PI, Brazil. thalesram@hotmail.com.ORCID http://orcid.org/0009-0004-5063-0606
Vinicius de Paula TaffarelCampus Professora Cinobelina Elvas, Federal University of Piauí, Bom Jesus, 64900-000, PI, Brazil.ORCID http://orcid.org/0009-0002-8228-976X
Gabriel de Araújo Silva CiprianoCampus Professora Cinobelina Elvas, Federal University of Piauí, Bom Jesus, 64900-000, PI, Brazil.ORCID http://orcid.org/0009-0004-2637-5481
Ionnara Diogo XavierCampus Professora Cinobelina Elvas, Federal University of Piauí, Bom Jesus, 64900-000, PI, Brazil.ORCID http://orcid.org/0009-0005-2160-9354
Yanka Manoelly Dos Santos GasparCampus Professora Cinobelina Elvas, Federal University of Piauí, Bom Jesus, 64900-000, PI, Brazil.ORCID http://orcid.org/0009-0000-5855-3183
Luysa Valéria Leal Coêlho RamosCampus Professora Cinobelina Elvas, Federal University of Piauí, Bom Jesus, 64900-000, PI, Brazil.ORCID http://orcid.org/0009-0004-8117-8771
Augusto Matias de OliveiraUniversity of Rio Verde, Rio Verde, 75901-970, GO, Brazil.ORCID http://orcid.org/0000-0003-3556-2030
Marcos Antonio Barbosa de LimaDepartment of Biology, Federal Rural University of Pernambuco, Recife, 52171-900, PE, Brazil.ORCID http://orcid.org/0000-0001-5987-224X
Galba Maria de Campos-TakakiCenter for Research in Environmental Sciences, Department of Chemistry, Catholic University of Pernambuco, Recife, 50050-900, PE, Brazil.ORCID http://orcid.org/0000-0002-0519-0849
Tiago de Oliveira SousaCampus Professora Cinobelina Elvas, Federal University of Piauí, Bom Jesus, 64900-000, PI, Brazil.ORCID http://orcid.org/0000-0001-7855-348X
Thiago Pajeú NascimentoCampus Professora Cinobelina Elvas, Federal University of Piauí, Bom Jesus, 64900-000, PI, Brazil.ORCID http://orcid.org/0000-0003-3480-6734
Alice Maria Gonçalves SantosCampus Professora Cinobelina Elvas, Federal University of Piauí, Bom Jesus, 64900-000, PI, Brazil.ORCID http://orcid.org/0000-0003-0672-1709

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The application of Trichoderma species as biological control agents requires stable formulations capable of preserving viability and ensuring consistent field efficacy. This study aimed to assess the stability and bio-efficacy of sodium alginate capsules containing conidia of Trichoderma species native to the Cerrado region of Piauí, Brazil. Five encapsulated species were evaluated for conidial viability after 15 months of storage, release profiles under different pH conditions, in vitro antagonistic activity against Fusarium verticillioides, and in vivo growth performance in different substrates. The capsules maintained structural integrity and viability above 1 × 10⁷ CFU mL⁻¹ for all species, with Trichoderma sp. 1 and Trichoderma sp. 2 exhibiting significant increases in conidial concentration over time. Conidial release was influenced by pH and exposure time, with the highest release observed at pH 7.0 for T. longibrachiatum and T. koningiopsis, while Trichoderma sp. 1 performed best at pH 5.0. In the antagonism assays, all species inhibited the growth of F. verticillioides, with T. longibrachiatum showing the strongest effect (45.97% inhibition). In in vivo bioassays, efficient colonization was observed across all tested substrates (soil, soil mixed with commercial substrate, and commercial substrate alone), with visible sporulation within 72 h. Trichoderma sp. 1 demonstrated the highest stability across substrates, whereas T. koningiopsis grew exclusively on commercial substrate. Collectively, these findings demonstrate that sodium alginate encapsulation effectively preserves the viability, structural stability, and biocontrol functionality of Trichoderma spp., underscoring its potential for development into commercial formulations suitable for diverse agricultural systems.

Indexed as

AlginatesBiological Control AgentsSpores, FungalTrichodermaAntibiosisBrazilFusariumGlucuronic AcidHexuronic AcidsHydrogen-Ion ConcentrationMicrobial ViabilitySoil MicrobiologyAlginatesBiological Control AgentsGlucuronic AcidHexuronic AcidsBiological ControlBiotechnologyConidial viabilityPH-dependent releaseSodium alginate capsules

Identifiers

PMID42113344
PMCPMC13161278

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.