Evidence map›Paper›PMID 42112781›Full record

ArticleEnvironmental and molecular mutagenesis2026

Analysis of Mutagenesis in the Escherichia coli tdk Gene: The Nucleoid Protein HNS Is Required for a Dramatic Cisplatin-Induced Hotspot.

Dana Sorensen, Tara Mata, Ananya Sridharan, Mallika Mathew, Emily Sagastume, Natasha Sutkin, Katherine Douglas, Mila Daniel, Angela Hung, Alina Garmash and 3 more

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Article in Environmental and molecular mutagenesis, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Dana SorensenDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Tara MataDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Ananya SridharanDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Mallika MathewDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Emily SagastumeDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Natasha SutkinDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Katherine DouglasDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Mila DanielDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Angela HungDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Alina GarmashDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Annette HsiehDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Jamie Elizabeth OrtegaDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Jeffrey H MillerDepartment of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.ORCID 0000-0002-6198-9057

Funding

University of California
6 · The paper itself

Abstract

We demonstrate the utility of the tdk reporter gene system, by showing its ability to not only analyze mutational hotspots, but also to allow the analysis of both weak and strong mutator or mutagen effects. It can detect large and small insertions and deletions, as well as base substitutions. We previously defined cisplatin (CPT)-induced hotspots in tdk. One extraordinary hotspot for G:C- > T:A transversions at base pair (bp) 499 is in a region of the gene that appears to be mutationally prone, suggesting that the conformation of the DNA in that region may play a major role in elevating mutation rates. Here, we examined CPT-induced mutations in a derivative of the starting wild-type strain that lacks the nucleoid binding protein HNS, which is involved in the folding and compaction of the E. coli chromosome. In this strain background the extraordinary CPT-induced hotspot disappears, pinpointing the importance of DNA conformation in mutation rates at certain positions. We further show that this A:T-rich gene is a magnet for transposable elements.

Indexed as

Bacterial ProteinsDNA-Binding ProteinsEscherichia coliEscherichia coli ProteinsThymidine KinaseCisplatinDNA Transposable ElementsGenes, BacterialGenes, ReporterINDEL MutationMutagenesisBacterial ProteinsCisplatinDNA-Binding ProteinsDNA Transposable ElementsEscherichia coli ProteinsH-NS protein, bacteriaThymidine Kinasebacterial systemsmutational hotspotstdk

Identifiers

PMID42112781
PMCPMC13159517

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.