ArticleFrontiers in microbiology2026
Culture, qPCR, and genome-based surveillance of
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
12 authors.
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Abstract
Background: Antimicrobial resistance (AMR) surveillance remains predominantly centered on clinical settings, potentially underestimating the community circulation of high-priority carbapenemase determinants. Methods: We integrated culture-based isolation, absolute qPCR, and whole-genome sequencing (WGS) to monitor Results: Total bacterial recovery on antibiotic-free control plates was comparable across seasons, supporting consistent sampling performance. Resistance rates peaked in winter for both ceftazidime (3.7% ± 0.87) and ciprofloxacin (11.1% ± 1.72). Across seasons, 92 morphotypes were recovered, dominated by the genera Conclusions: School wastewater provided a sensitive community sentinel matrix for targeted carbapenemase surveillance, and
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