ArticleFrontiers in microbiology2026
PEG-mediated transformation: a tool for random integration or targeted gene replacement in
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Genome Editing Approaches in Flax (International journal of molecular sciences · 2026Review
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Authors and funding
9 authors.
Funding
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Abstract
Introduction: Methods: In this study, polyethylene glycol (PEG)-mediated protoplast transformation was optimized for genome modification in Results: The optimized enzymatic digestion protocol yielded a high quantity of viable protoplasts, which was critical for successful transformation. Fluorescence microscopy confirmed stable RFP expression across five consecutive generations, during conidial germination on hydrophobic surfaces, and throughout the infection process on tea leaves, demonstrating the heritability and stability of transgene expression. Furthermore, targeted disruption of the cutinase gene showed that it had no effect on the pathogenicity of Discussion: These findings demonstrate that PEG-mediated transformation is an efficient and versatile approach for both random insertional mutagenesis and targeted gene deletion in
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