ArticleFrontiers in immunology2026
Comparative evaluation of performance and precision of multiplex immunoassays measuring human IgG to
Article in Frontiers in immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Methods: The MSD assay platform employs positional multiplexing with electrochemiluminescent detection, while the Luminex system uses bead-based multiplexing with fluorescent detection. Both assays used standardized intravenous immunoglobulin dilutions as reference standards. Assay precision was evaluated by analyzing eight samples from human volunteers selected to span the dynamic range for the four antigens Streptolysin O, Results: Both platforms demonstrated comparable intermediate precision and repeatability profiles. Increased variance was observed in samples with very low antibody concentrations. Neither operator variability nor day-to-day factors significantly impacted assay results. Additionally, inter-assay coefficients of variation for standards remained below 15% within the pre-established quantitative ranges for both platforms. Discussion: The findings demonstrate that both multiplex immunoassays show equivalent precision and repeatability for quantifying human IgG to four Strep A vaccine antigens, making both platforms suitable for supporting early clinical trials and seroepidemiological studies.
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