ArticlemicroPublication biology2026
Long-term cultivation shifts transcriptomic profile of HCT-116 cells.
Article in microPublication biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Adaptation of Non-Invasive Cancer Cells to 3D Collagen I Microenvironment Induces Transcriptional Reprogramming Accompanied by a More Complex RNA Landscape.International journal of molecular sciences · 2026Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human cancer cell lines are widely used in artificial culture systems to study cancer biology due to their immortalization, enabling extended culturing compared to primary cells. However, genomic instability can rapidly alter cellular behavior, raising questions about the transcriptome stability for controlled studies. We cultivated HCT-116 cells over 25 passages (~16 weeks), and observed a stable transcriptome up to passage 20, with only minimal differential gene expression. Above passage 20, genes corresponding to cell proliferation and cell death were upregulated suggesting a change in general cellular regulation and underlining defined cell culture time periods for stable and reproducible experimental conditions.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.