ArticlePeerJ2026
Response of soil bacterial community structure in Pisha sandstone areas to different stumping.
Article in PeerJ, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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8 authors.
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Abstract
Background: The Pisha sandstone region of Inner Mongolia is one of the areas most severely affected by soil erosion in the world. Stumping is a widely used practice for rejuvenating degraded sea buckthorn ( Methods: We conducted a field experiment in a 10-year-old declining sea buckthorn plantation, applying four stumping heights (0 cm, 10 cm, 15 cm, 20 cm) with uncut forest as control. Soil samples were collected from three compartments (root surface, rhizosphere, non-rhizosphere). Bacterial community composition was characterized by high-throughput sequencing of the 16S rRNA gene V4 region using primers 515F/806R with high-fidelity DNA polymerase. Soil physicochemical properties were measured using standard methods. Results: Stumping at 15 cm height consistently exhibited the strongest effects across all soil compartments. Compared to other treatments and the control, this treatment significantly increased soil organic carbon, available nitrogen, phosphorus, and potassium, and enhanced bacterial α-diversity (Chao1, Shannon). At the family level, 15 cm stumping most strongly enriched
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