Evidence map›Paper›PMID 42111707›Full record

ArticleBio-protocol2026

An Advanced Single-Cell RNA Sequencing (scRNA-seq) Protocol Utilizing Custom-Designed Multiplexing.

Feng Gao, Xujie Liu, Fan Sun, Yadong Xiao, Gutian Xiao, Zhaoxia Qu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Feng GaoDepartment of Immunology and Immune Therapeutics, Hastings Center for Pulmonary Research, Norris Comprehensive Cancer Center, University of Southern California Keck School of Medicine, Los Angeles, CA, USA.
Xujie LiuDepartment of Immunology and Immune Therapeutics, Hastings Center for Pulmonary Research, Norris Comprehensive Cancer Center, University of Southern California Keck School of Medicine, Los Angeles, CA, USA.
Fan SunDepartment of Microbiology and Molecular Genetics, UPMC Hillman Cancer Center, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Yadong XiaoDepartment of Immunology and Immune Therapeutics, Hastings Center for Pulmonary Research, Norris Comprehensive Cancer Center, University of Southern California Keck School of Medicine, Los Angeles, CA, USA.
Gutian XiaoDepartment of Immunology and Immune Therapeutics, Hastings Center for Pulmonary Research, Norris Comprehensive Cancer Center, University of Southern California Keck School of Medicine, Los Angeles, CA, USA.
Zhaoxia QuDepartment of Immunology and Immune Therapeutics, Hastings Center for Pulmonary Research, Norris Comprehensive Cancer Center, University of Southern California Keck School of Medicine, Los Angeles, CA, USA.

Funding

Structural and molecular dissection of NF-kappaB regulation by the ubiquitin E3 ligase PDLIM2 in lung innate immunity and diseasesR01GM144890 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI QU, ZHAOXIA, XIAO, GUTIAN · 2022 to 2025
$2.1M
Mechanistic difference of NF-kappaB in lung physiology and tumorigenesisR01CA258614 · NCI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Zhaoxia Qu, GUTIAN XIAO · 2022 to 2026
$1.9M
The NF-kappaB modulator PDLIM2 as an intrinsic checkpoint of alveolar macrophages critical for lung innate immunity, inflammation and diseasesR01HL177140 · NHLBI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Zhaoxia Qu · 2025 to 2026
$1.6M
NCI NIH HHS R01 CA258614NHLBI NIH HHS R01 HL177140NIGMS NIH HHS R01 GM144890
6 · The paper itself

Abstract

While cell hashing enhances single-cell RNA sequencing (scRNA-seq) efficiency and minimizes batch effects, commercial mouse hashtags often fail in FVB/N and several other strains due to antibody-epitope incompatibility. We describe a robust alternative utilizing biotinylated antibody cocktails and streptavidin-conjugated oligos to enable reliable sample multiplexing. This approach was validated in FVB/N lung tissues, yielding high-quality single-cell libraries. Our protocol offers a practical solution for researchers requiring strain-specific or custom-designed multiplexing strategies for single-cell transcriptomics. Key features • Strain-specific compatibility: Resolves the known H-2

Indexed as

5′ gene expressionBiotin-streptavidin conjugationCell hashingFVB/N miceMHC-I haplotype mismatchSample multiplexingscRNA-seq

Identifiers

PMID42111707
PMCPMC13156703

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.