Visualization of Immunological Synapse Formation by Intercellular Cross-Linking of T Cell-Dependent Bispecific Antibodies Using Fluorescence Microscopy.

Rikuto Nakamura, Ryo Tsumura, Takahiro Anzai, Ryutaro Asano, Masahiro Yasunaga

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Rikuto NakamuraDivision of Developmental Therapeutics, EPOC, National Cancer Center, Kashiwa, Chiba, 277-8577, Japan.

Ryo TsumuraDivision of Developmental Therapeutics, EPOC, National Cancer Center, Kashiwa, Chiba, 277-8577, Japan.

Takahiro AnzaiDivision of Developmental Therapeutics, EPOC, National Cancer Center, Kashiwa, Chiba, 277-8577, Japan.

Ryutaro AsanoDepartment of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.

Masahiro YasunagaDivision of Developmental Therapeutics, EPOC, National Cancer Center, Kashiwa, Chiba, 277-8577, Japan. mayasuna@east.ncc.go.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immunocytochemistry is a common method to visually detect the localization of target proteins in cells or tissues using fluorescent dyes conjugated to antibodies. Especially in the field of cancer therapeutics, it is used to visualize and analyze the localization of target proteins in cancer cells and their interactions with other immune cells. In this chapter, we describe a protocol to detect how T cell-dependent bispecific antibodies (TDBs) cross-link cancer cells and T cells. We focus on the adhesion molecules involved in the cross-linking between cells by TDBs and provide a quick and easy method for visualization.

Indexed as

Antibodies, BispecificImmunological SynapsesT-LymphocytesCell CommunicationCell LineCoculture TechniquesHumansMicroscopy, FluorescenceAntibodies, BispecificBispecific antibodyImmunocytochemistryImmunological synapseMicroscopy

Identifiers

PMID42108347

DOI10.3390/ph14111172

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.