Development of a quality control test for diphtheria vaccine based on an electrochemical aptamer-based biosensor.

Abrar Almusharraf, Amina Rhouati, Dana Cialla-May, Jürgen Popp, Mohammed Zourob

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Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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5 authors.

Abrar AlmusharrafDepartment of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Rd, Riyadh, 11355, Saudi Arabia.

Amina RhouatiDepartment of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Rd, Riyadh, 11355, Saudi Arabia.

Dana Cialla-MayInstitute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich Schiller University Jena, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Helmholtzweg 4, 07743, Jena, Germany.

Jürgen PoppInstitute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich Schiller University Jena, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Helmholtzweg 4, 07743, Jena, Germany.

Mohammed ZourobDepartment of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Rd, Riyadh, 11355, Saudi Arabia. mzourob@alfaisal.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

At every stage of the vaccine's development, including research and development together with the routine production (in-process control of batch release), quality control testing is a crucial operation for ensuring the efficacy, safety and consistency of final products. Conventional quality control tests rely on a combination of biochemical, physicochemical, and cell-based assays. In this study, we report a quality control test based on an electrochemical aptamer-based sensor with a specific application to Diphtheria vaccine. First, specific aptamers for Diphtheria are successfully selected and characterised by using SELEX (Systematic Evolution of Ligands by EXponential enrichment) process. A total of 11 rounds were performed to select five sequences with variable affinities to Diphtheria toxoid. The aptamer D1 exhibiting the best affinity towards the Diphtheria toxoid (K

Indexed as

Aptamers, NucleotideBiosensing TechniquesDiphtheria ToxoidElectrochemical TechniquesElectrodesHumansQuality ControlSELEX Aptamer TechniqueAptamers, NucleotideDiphtheria ToxoidAptamerDiphtheriaElectrochemistryQuality control testingSELEXVaccines

Identifiers

PMID42108308

DOI10.1038/s41598-025-33003-9

PMCPMC13346428

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.