Evidence map›Paper›PMID 42107044›Full record

ArticleSTAR protocols2026

Protocol for proximity ligation assay combined with immunolabeling of paraffin-embedded lung tissue in a porcine model.

Aurélie Bompard, Robin Regulus, Alexandre Cousin, Cyril Salama, Audrey Lejart, Laure Barbier, Diane Riccobono, Anne-Laure Favier, Krisztina Nikovics

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Aurélie BompardImaging Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, Brétigny-sur-Orge 91223, France.
Robin RegulusImaging Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, Brétigny-sur-Orge 91223, France.
Alexandre CousinImaging Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, Brétigny-sur-Orge 91223, France.
Cyril SalamaImaging Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, Brétigny-sur-Orge 91223, France.
Audrey LejartMolecular Biology Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, Brétigny-sur-Orge 91223, France.
Laure BarbierMolecular Biology Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, Brétigny-sur-Orge 91223, France.
Diane RiccobonoRadiobiology Unit, Department of NRBC Defense, French Armed Forces Biomedical Research Institute, Brétigny-sur-Orge 91223, France.
Anne-Laure FavierImaging Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, Brétigny-sur-Orge 91223, France.
Krisztina NikovicsImaging Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, Brétigny-sur-Orge 91223, France. Electronic address: krisztina.nikovics@def.gouv.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein-protein interaction (PPI) studies are essential for understanding cellular communication, and identifying the specific cell types in which these interactions occur is critical. Here, we present a protocol for detecting protein proximities in a porcine model using the proximity ligation assay (PLA) combined with immunolabeling to assign signals to defined cell populations. We describe the steps for slide preparation, including deparaffinization, heat-induced epitope retrieval (HIER), permeabilization, blocking of sites, and incubation with primary antibodies. We also detail the procedures for combining the PLA method with immunolabeling. For complete details on the use and execution of this protocol, please refer to Cousin et al.

Indexed as

ImmunohistochemistryLungParaffin EmbeddingProtein Interaction MappingAnimalsSwineAntibodyCell BiologyGene ExpressionIn Situ Hybridization

Identifiers

PMID42107044
PMCPMC13188080

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.