Evidence map›Paper›PMID 42105746›Full record

ArticleCell reports methods2026

In vivo visualization of bioactive iron and oxygen using LiON, the labile iron and oxygen notifier.

Ayato Maeda, Akihiro Nita, Shoko Sashiyama, Suzu Yoshitomo, Komen Joan Jepkosgei, Yuqing Xu, Yuichiro Arima, Keiichi I Nakayama, Kimi Araki, Toshiro Moroishi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ayato MaedaDivision of Cellular Dynamics, Medical Research Laboratory, Institute of Integrated Research, Institute of Science Tokyo, 1-5-45 Yushima, Tokyo 113-8510, Japan; Department of Molecular and Medical Pharmacology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
Akihiro NitaDivision of Cellular Dynamics, Medical Research Laboratory, Institute of Integrated Research, Institute of Science Tokyo, 1-5-45 Yushima, Tokyo 113-8510, Japan.
Shoko SashiyamaDivision of Cellular Dynamics, Medical Research Laboratory, Institute of Integrated Research, Institute of Science Tokyo, 1-5-45 Yushima, Tokyo 113-8510, Japan; Department of Molecular and Medical Pharmacology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
Suzu YoshitomoDivision of Cellular Dynamics, Medical Research Laboratory, Institute of Integrated Research, Institute of Science Tokyo, 1-5-45 Yushima, Tokyo 113-8510, Japan; Department of Molecular and Medical Pharmacology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
Komen Joan JepkosgeiDivision of Cellular Dynamics, Medical Research Laboratory, Institute of Integrated Research, Institute of Science Tokyo, 1-5-45 Yushima, Tokyo 113-8510, Japan; Department of Molecular and Medical Pharmacology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
Yuqing XuDevelopmental Cardiology Laboratory, International Research Center for Medical Sciences (IRCMS), Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.
Yuichiro ArimaDevelopmental Cardiology Laboratory, International Research Center for Medical Sciences (IRCMS), Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.
Keiichi I NakayamaAnticancer Strategies Laboratory, Advanced Research Initiative, Institute of Integrated Research, Institute of Science Tokyo, 1-5-45 Yushima, Tokyo 113-8510, Japan.
Kimi ArakiInstitute of Resource Development and Analysis, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.
Toshiro MoroishiDivision of Cellular Dynamics, Medical Research Laboratory, Institute of Integrated Research, Institute of Science Tokyo, 1-5-45 Yushima, Tokyo 113-8510, Japan; Department of Molecular and Medical Pharmacology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan. Electronic address: moroishi.toshiro@tmd.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Iron and oxygen are essential for numerous biological processes, yet monitoring their bioactive intracellular dynamics in vivo remains challenging due to the limitations of current detection methods. Here, we introduce the labile iron and oxygen notifier (LiON), a genetically encoded, ratiometric fluorescent reporter enabling single-cell-resolution monitoring of intracellular iron and oxygen levels in vivo. In cultured cells, LiON responds dynamically to physiologically relevant changes in iron and oxygen levels. Using LiON-knockin mice, we revealed heterogeneous distribution of iron and oxygen across tissues, cell types, and even among individual cells of the same type. Thus, LiON offers a platform for studying iron and oxygen metabolism in living organisms, providing insights into the cellular diversity of iron and oxygen utilization and their roles in physiology and disease.

Indexed as

IronOxygenAnimalsHumansMiceIronOxygenbioactive ironCP: imaginggenetically encoded reporteriron homeostasisoxygen dynamicssingle-cell imaging

Identifiers

PMID42105746
PMCPMC13282663

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.