Evidence map›Paper›PMID 42105252›Full record

ArticleJournal of animal science2026

Effects of dietary starch concentration and feeding management regimen on bacterial pathogens in liver abscesses and ruminal and colonic epithelial tissues in feedlot steers.

Kasi N Schneid, Mina Abbasi, J Daniel Young, Alyssa K Deters, Xiaorong Shi, Leigh Ann George, T G Nagaraja, Ty E Lawrence, John T Richeson, Kendall L Samuelson

Abstract read
In one paragraph

Article in Journal of animal science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kasi N SchneidDepartment of Agricultural Sciences, West Texas A&M University, Canyon, TX 79016, United States.
Mina AbbasiDepartment of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS 66506, United States.
J Daniel YoungDepartment of Agricultural Sciences, West Texas A&M University, Canyon, TX 79016, United States.ORCID 0009-0000-4085-1158
Alyssa K DetersDepartment of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS 66506, United States.
Xiaorong ShiDepartment of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS 66506, United States.
Leigh Ann GeorgeDepartment of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS 66506, United States.
T G NagarajaDepartment of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS 66506, United States.ORCID 0000-0002-0899-878X
Ty E LawrenceDepartment of Agricultural Sciences, West Texas A&M University, Canyon, TX 79016, United States.ORCID 0000-0001-7088-5455
John T RichesonDepartment of Agricultural Sciences, West Texas A&M University, Canyon, TX 79016, United States.ORCID 0000-0002-6632-8557
Kendall L SamuelsonDepartment of Agricultural Sciences, West Texas A&M University, Canyon, TX 79016, United States.ORCID 0000-0001-7116-0173

Funding

Foundation for Food and Agriculture Research and International Consortium for Antimicrobial Stewardship in Agriculture ICASALAWG-064
6 · The paper itself

Abstract

This study evaluated the main and interactive effects of dietary starch concentration (49.1% [control] or 64.4% [high starch]) and feeding management regimen (regular [REG] or erratic [ERR]) on prevalence of Fusobacterium necrophorum subsp. necrophorum, F. necrophorum subsp. funduliforme, Trueperella pyogenes, and Salmonella enterica in liver abscesses (LA) and ruminal and colonic tissues of finishing beef steers. Intact LA (n = 132) and sections of ruminal (n = 131) and colonic (n = 120) epithelial tissues were collected from steers at slaughter. Samples were cultured, before and after enrichment, to determine prevalence of four bacterial pathogens and quantitative PCR (qPCR) was used to determine prevalence and concentrations of the two subspecies of F. necrophorum. Subsp. necrophorum was the dominant pathogen in LA, whereas subsp. funduliforme was dominant in ruminal and colonic tissues by both culture and qPCR methods. Steers fed the high-starch diet had greater prevalence (P = 0.01) of subsp. necrophorum, T. pyogenes, and S. enterica in LA compared to steers fed the control diet, indicating the potential impact of dietary starch on promoting bacterial translocation. Of the 132 LA analyzed, culture and qPCR identified 21.7% and 16.4% of LA, respectively, to have subsp. funduliforme, without the subsp. necrophorum, suggesting that subsp. funduliforme without subsp. necrophorum may have caused LA. Concentrations of F. necrophorum in LA were between 7.0 to 7.5 log10 CFU per g, were 2 to 3 log less in ruminal and colonic epithelial tissues, and were not affected by the diet (P ≥ 0.08). The prevalence of S. enterica was greater (P < 0.01) in colonic (71.9%) than ruminal epithelial tissues (48.3%), suggesting that the colon may be the major source of the Salmonella in LA. A total of eight serotypes were identified, and the majority (88.6%) belonged to Kentucky (27.2%), Montevideo (26.2%), Anatum (23.3%), and Lubbock (11.9%). Feeding management regimen had minimal effect, however, diet affected prevalence of the pathogens in LA but not ruminal and colonic epithelial tissues. The prevalence and concentrations of both subspecies of F. necrophorum in colonic epithelial tissue suggest the possibility of the colon being a source of LA pathogens. However, the prevalence and concentration of F. necrophorum in colonic epithelial tissues were much less than that of ruminal tissues, suggesting that the rumen may be the primary reservoir of F. necrophorum in LA.

Indexed as

Cattle DiseasesDietary CarbohydratesLiver AbscessRumenStarchAnimal FeedAnimal Nutritional Physiological PhenomenaAnimalsCattleColonDietMaleSalmonella entericaDietary CarbohydratesStarchcattlediet compositionerratic feedingFusobacterium necrophorumpathogenesis

Identifiers

PMID42105252
PMCPMC13293063

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.