ArticleSTAR protocols2026
Protocol for ex vivo culture of neonate mouse ovaries to study early follicle activation.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
3 authors.
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Abstract
Establishing an ex vivo culture of neonatal ovaries enables controlled investigation of early follicle dynamics. Here, we present a protocol for isolating ovaries from neonatal female mice for maintaining intact ovarian architecture by ex vivo culture on a membrane allowing access to defined medium. We describe procedures for media preparation, ovary dissection, culture setup, and follicle development by stereomicroscopy and histological assessment. This protocol provides a platform for examining the regulation of the primordial-to-primary transition and growth. For complete details on the use and execution of this protocol, please refer to Amoushahi et al.
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Registered trials
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