Evidence map›Paper›PMID 42104005›Full record

ArticleApplied microbiology and biotechnology2026

Polycationic nanopeptide-fused endolysins for the control of Mannheimia haemolytica.

Sidra Moqaddes, Hechao Du, Jieting Lin, Jae Eun Hyun, Mawra Gohar, Alexei Savchenko, Kim Stanford, Brenda Ralston, Tim A McAllister, Yan Dong Niu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Sidra MoqaddesFaculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.
Hechao DuCollege of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing, Jiangsu, China.
Jieting LinFaculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.
Jae Eun HyunFaculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.
Mawra GoharFaculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.
Alexei SavchenkoCumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Kim StanfordBiological Science, University of Lethbridge, Lethbridge, AB, Canada.
Brenda RalstonLakeland College, Vermillion, AB, Canada.
Tim A McAllisterLethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada.
Yan Dong NiuFaculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada. dongyan.niu@ucalgary.ca.

Funding

Results Driven Agriculture Research 2019R052Rthe National Natural Science Foundation of China 32202003
6 · The paper itself

Abstract

Bovine respiratory disease (BRD) remains a major economic and animal health concern in cattle production, exacerbated by rising antimicrobial resistance and limited vaccine efficacy. Endolysins, phage-derived peptidoglycan hydrolases, have emerged as promising alternatives to antibiotics, but their activity against gram-negative bacteria is hindered by the outer membrane (OM) barrier. In this study, we identified five distinct prophage-derived endolysins from Mannheimia haemolytica and successfully expressed two (185 and 587AP2) along with their engineered polycationic nanopeptide (PCNP) fusions (PCNP-185 and PCNP-587AP2). All four recombinant proteins demonstrated muralytic activity, with original endolysins exhibiting higher enzymatic efficiency. However, PCNP-fused variants showed superior antibacterial effects as compared to those without. Especially, PCNP-185 achieved the most consistent multi-log

Indexed as

Anti-Bacterial AgentsEndopeptidasesMannheimia haemolyticaAnimalsCattleEdetic AcidPolyelectrolytesProphagesRecombinant Fusion ProteinsAnti-Bacterial AgentsEdetic AcidendolysinEndopeptidasesPolyelectrolytesRecombinant Fusion ProteinsAntibacterial activityBacteriophage endolysinBovine respiratory diseaseEDTAM. haemolyticaPolycationic nanopeptide

Identifiers

PMID42104005
PMCPMC13236837

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.