Evidence map›Paper›PMID 42103858›Full record

ArticleScientific reports2026

Detection of papillomavirus DNA in uterine flushing samples from healthy mares reveals diverse genotypes with differing host ranges.

Mariassunta Claudia D'Amato, Anna Cutarelli, Edoardo Vigetti, Tommaso di Palma, Serena Montagnaro, Fabio Del Piero, Michele Napoletano, Giovanna Fusco, Sante Roperto

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Mariassunta Claudia D'AmatoDipartimento di Medicina Veterinaria e delle Produzioni Animali, Università degli Studi di Napoli Federico II, Napoli, Italy.
Anna CutarelliIstituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Italy.
Edoardo VigettiDipartimento Agricoltura e Sviluppo Rurale, Regione Calabria, Catanzaro, Italy.
Tommaso di PalmaMedico Veterinario Libero Professionista, Ruoti, Italy.
Serena MontagnaroDipartimento di Medicina Veterinaria e delle Produzioni Animali, Università degli Studi di Napoli Federico II, Napoli, Italy.
Fabio Del PieroDepartment of Pathobiological Sciences and LSU Diagnostics - LADDL, School of Veterinary Medicine, Louisiana State University, Baton Rouge, USA.
Michele NapoletanoIstituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Italy.
Giovanna FuscoIstituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, Italy.
Sante RopertoDipartimento di Scienze Cliniche e Medicina Traslazionale, Università degli Studi di Roma "Tor Vergata", Rome, Italy. sante.roperto@uniroma2.it.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A molecular study to assess the presence of some papillomaviruses was performed on 105 uterine flushing samples of healthy mares using real time quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR). Bovine, equine, and ovine papillomavirus DNA was detected in 27 uterine flushing samples through dPCR (~ 25.71%); qPCR was able to detect 18 positive samples (~ 17.14%). Differences between the two molecular protocols were significant as shown by McNemar's test (p˂ 0.005). EcPV DNA was detected in 10 samples by dPCR (~ 9.52%) of uterine flushing samples. qPCR detected EcPV DNA in 7 samples (~ 6.66). dPCR revealed OaPV1 DNA in 13 samples (12.38%). qPCR revealed OaPV1 DNA in 9 samples (8.57%). BPV DNA was found by dPCR in 4 samples (~ 3.80%); qPCR revealed BPV DNA in two uterine flushing samples, that is ~ 1.90% of healthy uterine flushing samples. Overall, OaPV1 was the most detected papillomavirus from mare uterus. Similar molecular findings were found in the virobiota of mare vagina. EcPV9 was the most prevalent equine PV genotype. EcPV8, BPV13, ChPV1, ChPV2, OaPV2, OaPV3, and OaPV4 were not detected. Our findings are consistent with non-strict host specificity of BPVs and OaPVs.

Indexed as

DNA, ViralHost SpecificityPapillomaviridaePapillomavirus InfectionsUterusAnimalsCattleFemaleGenotypeHorsesReal-Time Polymerase Chain ReactionSheepDNA, ViralBovine papillomavirusDigital PCR (dPCR)Equine papillomavirusOvine papillomavirusReal time quantitative polymerase chain reaction (qPCR)Uterine flushing

Identifiers

PMID42103858
PMCPMC13342596

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.