NS5 polymerase stimulates RNA duplex unwinding by the Orthoflavivirus NS3 helicase.

Jamie J Arnold, Shubeena Chib, Craig E Cameron

Abstract read

In one paragraph

Article in The Journal of biological chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jamie J ArnoldDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA. Electronic address: jamie_arnold@med.unc.edu.

Shubeena ChibDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA.

Craig E CameronDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA. Electronic address: craig.cameron@unc.edu.

Funding

RNA DEPENDENT RNA POLYMERASE MECHANISMR01AI045818 · NIAID · UNIV OF NORTH CAROLINA CHAPEL HILL · PI ARNOLD, JAMIE JON, CAMERON, CRAIG E. · 1999 to 2025

$7.5M

NIAID NIH HHS R01 AI045818

6 · The paper itself

Abstract

The NS3 helicases from the Flaviviridae family of viruses exhibit nucleotide-hydrolysis-dependent, nucleic-acid-unwinding activity. The RNA unwinding activity for NS3 helicases from the Orthoflavivirus genus has not been fully explored and contrasts with NS3 helicase from hepatitis C virus (HCV) of the Hepacivirus genus, which has thus far served as the prototypical model enzyme from this family of viruses. To begin to understand the functional differences between flavivirus NS3 helicases, we first developed an expression and purification system for full-length untagged NS3 protein from West Nile virus and Zika virus. Both enzymes exhibit RNA-stimulated ATPase activity and are dependent on the nucleoside triphosphatase active site of the enzyme. Unlike HCV NS3, Orthoflavivirus NS3s do not efficiently pre-assemble on a 3'-ssRNA-tailed dsRNA substrate in the absence of Mg

Indexed as

RNA-Dependent RNA PolymeraseRNA, Double-StrandedRNA HelicasesViral Nonstructural ProteinsWest Nile virusZika VirusDEAD-box RNA HelicasesNucleoside-TriphosphataseSerine EndopeptidasesViral ProteasesDEAD-box RNA HelicasesNS3 protein, flavivirusNS5 protein, flavivirusNucleoside-TriphosphataseRNA-Dependent RNA PolymeraseRNA, Double-StrandedRNA HelicasesSerine EndopeptidasesViral Nonstructural ProteinsViral ProteaseshelicaseNS3NS5NTPaseOrthoflavivirusRNA-dependent RNA polymeraseRNA unwindingRNA virus

Identifiers

PMID42103212

DOI10.1016/j.jbc.2026.113118

PMCPMC13254602

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.